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European Radiology

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22-02-2022 | Glioblastoma | Molecular Imaging

MGMT promoter methylation status shows no effect on [¹⁸F]FET uptake and CBF in gliomas: a stereotactic image-based histological validation study

Authors: Shuangshuang Song, Yi Shan, Leiming Wang, Ye Cheng, Hongwei Yang, Guoguang Zhao, Zhenguang Wang, Jie Lu

Published in: European Radiology | Issue 8/2022

Abstract

Objectives

To investigate the effects of O⁶-methylguanine DNA methyltransferase (MGMT) promoter methylation status of gliomas on O-(2-¹⁸F-fluoroethyl)-L-tyrosine ([¹⁸F]FET) uptake and cerebral blood flow (CBF) of arterial spin labeling (ASL), evaluated by hybrid PET/MR. Stereotactic biopsy was used to validate the findings.

Methods

A set of whole tumor and reference volumes of interest (VOIs) based on PET/FLAIR imaging were delineated and transferred to the corresponding [¹⁸F]FET PET and CBF maps in 57 patients with newly diagnosed gliomas. The mean and max tumor-to-brain ratio (TBR) and normalized CBF (nCBF) were calculated. The predictive efficacy of [¹⁸F]FET PET and CBF in determining MGMT promoter methylation status of glioma were evaluated by whole tumor analysis and stereotactic biopsy. The correlation between PET/MR parameters and MGMT promoter methylation were analyzed using histological specimens acquired from multiple stereotactic biopsies.

Results

Based on the analysis of whole tumor volume and biopsy site, TBRmean, TBRmax, nCBFmean, and nCBFmax showed no statistically significant differences between gliomas with and without MGMT promoter methylation (all p > 0.05). Furthermore, stereotactic biopsy demonstrated that TBRmean, TBRmax, nCBFmean, and nCBFmax showed no correlation with MGMT promoter methylation (r = −0.117, p = 0.579; r = −0.161, p = 0.443; r = −0.271, p = 0.191; r = −0.300, p = 0.145; respectively).

Conclusions

MGMT promoter methylation status shows no effect on [¹⁸F]FET uptake and CBF of ASL in gliomas. Stereotactic biopsy validates it and further reveals there is no correlation of [¹⁸F]FET PET uptake and CBF with the percentages of MGMT promoter methylation.

Key Points

• Based on whole tumor VOI assessment, MGMT promoter methylation status shows no effect on [ ¹⁸ F]FET uptake and CBF of ASL in gliomas.

• For WHO grade IV glioblastomas, [ ¹⁸ F]FET PET and ASL parameters based on hybrid PET/MR fail to predict the MGMT promoter methylation status.

• Stereotactic image–based histology reveals that there is no correlation of [ ¹⁸ F]FET PET uptake and CBF with the status and percentages of MGMT promoter methylation in gliomas.

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Title: MGMT promoter methylation status shows no effect on [18F]FET uptake and CBF in gliomas: a stereotactic image-based histological validation study
Authors: Shuangshuang Song
Yi Shan
Leiming Wang
Ye Cheng
Hongwei Yang
Guoguang Zhao
Zhenguang Wang
Jie Lu
Publication date: 22-02-2022
Publisher: Springer Berlin Heidelberg
Keywords: Glioblastoma
Astrocytoma
Glioma
Glioma
Astrocytoma
Glioblastoma
Positron Emission Tomography
Astrocytoma
Glioblastoma
Oligodendroglioma
Glioma
Published in: European Radiology / Issue 8/2022
Print ISSN: 0938-7994
Electronic ISSN: 1432-1084
DOI: https://doi.org/10.1007/s00330-022-08606-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

MGMT promoter methylation status shows no effect on [¹⁸F]FET uptake and CBF in gliomas: a stereotactic image-based histological validation study

Abstract

Objectives

Methods

Results

Conclusions

Key Points

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Methods

Results

Conclusions

Key Points

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