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Virology Journal
Published in: Virology Journal 1/2017

Open Access 01-12-2017 | Short Report

Duck enteritis virus (DEV) UL54 protein, a novel partner, interacts with DEV UL24 protein

Authors: Xinghong Gao, Renyong Jia, Mingshu Wang, Qiao Yang, Shun Chen, Mafeng Liu, Zhongqiong Yin, Anchun Cheng

Published in: Virology Journal | Issue 1/2017

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Abstract

Background

UL24 is a multifunctional protein that is conserved among alphaherpesviruses and is believed to play an important role in viral infection and replication.

Results

In this paper, to investigate putative UL24-binding proteins and to explore the functional mechanisms of DEV UL24, yeast two-hybrid (Y2H) was carried out, and further verified the interaction between UL24 and partners by co-immunoprecipitation and fluorescence microscopy experiments. Interaction partners of UL24 protein were screened by yeast two-hybrid (Y2H) with the cDNA library of DEV-CHv strain post-infection DEF cells. A novel partner, DEV UL54 protein, was discovered by Y2H screening and bioinformatic. Co-immunoprecipitation experiments suggested that DEV UL24 interacted with UL54 proteins. And distribution of a part of UL54 protein was changed from nucleus to cytoplasm in DF-1 cells of co-subcellular localization experiments which also showed that DEV UL24 interacted with UL54 proteins.

Conclusions

The interaction between the DEV UL24 and UL54 proteins was discovered for the first time. Thus, DEV UL54 protein as a novel partner interacted with DEV UL24 protein.
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Metadata
Title
Duck enteritis virus (DEV) UL54 protein, a novel partner, interacts with DEV UL24 protein
Authors
Xinghong Gao
Renyong Jia
Mingshu Wang
Qiao Yang
Shun Chen
Mafeng Liu
Zhongqiong Yin
Anchun Cheng
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2017
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/s12985-017-0830-5

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