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Virology Journal
Published in: Virology Journal 1/2010

Open Access 01-12-2010 | Short report

Expression and intracellular localization of duck enteritis virus pUL38 protein

Authors: Jun Xiang, Guangpeng Ma, Shunchuan Zhang, Anchun Cheng, Mingshu Wang, Dekang Zhu, Renyong Jia, Qihui Luo, Zhengli Chen, Xiaoyue Chen

Published in: Virology Journal | Issue 1/2010

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Abstract

Knowledge of the intracellular location of a protein can provide useful insights into its function. Bioinformatic studies have predicted that the DEV pUL38 mainly targets the cytoplasm and nucleus. In this study, we obtained anti-pUL38 polyclonal sera. These antibodies were functional in western blotting and immunofluorescence in DEV-infected duck embryo fibroblasts (DEFs). pUL38 was expressed as a 51-kDa protein from 8 h post-infection onward, initially showing a diffuse distribution throughout the cytoplasm, and later in the nucleus. Furthermore, pUL38 was found in purified virus. These results provide the first evidence of the kinetics of expression and intracellular localization of DEV pUL38.
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Metadata
Title
Expression and intracellular localization of duck enteritis virus pUL38 protein
Authors
Jun Xiang
Guangpeng Ma
Shunchuan Zhang
Anchun Cheng
Mingshu Wang
Dekang Zhu
Renyong Jia
Qihui Luo
Zhengli Chen
Xiaoyue Chen
Publication date
01-12-2010
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2010
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-7-162

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