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01-10-2019 | Hepatitis B | Original Paper

Analysis of hepatitis B virus-mixed genotype infection by ultra deep pyrosequencing in Sudanese patients, 2015–2016

Authors: Khalid Abdallah Enan, Claudia Minosse, Abdel Rahim Mohammed El Hussein, Marina Selleri, Emanuela Giombini, Maria Rosaria Capobianchi, Isam Mohamed Elkhidir, Mohamed Omer Mustafa, Osama Mohamed Khair, Dina Ahamed Hassan, Anna Rosa Garbuglia

Published in: Infection | Issue 5/2019

Abstract

Purpose

The frequency of detection of HBV co-infection with multiple HBV genotypes is influenced by the detection method; usually co-infections are detected by multiplex PCR or hybridization assays, and are rarely confirmed by sequencing and conventional cloning. The objective of this study was to confirm by ultra-deep pyrosequencing (UDPS) mixed HBV infections, previously detected by multiplex-nested PCR.

Methods

Sixteen samples from HBV co-infected Sudanese patients detected by multiplex-nested PCR, were amplified targeting the P/S region and sequenced by UDPS.

Results

The only genotypes identified using UDPS were D and E, while A, B, C and F genotypes, previously detected by multiplex-nested PCR, were not detected. Specifically, 10 samples were shown to be mono-infected (D or E); in 3 out of 10 mono-infected D patients, a subtype combination was observed: D1 + D7 in 2 cases and D2 + D6 in 1 case. The remaining 6 subjects were D + E co-infected (harboring different mixtures of D subtypes).

Conclusions

Overall, UDPS is more effective than multiplex-nested PCR for identifying multiple HBV genotypes and subtypes infections.

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Title: Analysis of hepatitis B virus-mixed genotype infection by ultra deep pyrosequencing in Sudanese patients, 2015–2016
Authors: Khalid Abdallah Enan
Claudia Minosse
Abdel Rahim Mohammed El Hussein
Marina Selleri
Emanuela Giombini
Maria Rosaria Capobianchi
Isam Mohamed Elkhidir
Mohamed Omer Mustafa
Osama Mohamed Khair
Dina Ahamed Hassan
Anna Rosa Garbuglia
Publication date: 01-10-2019
Publisher: Springer Berlin Heidelberg
Keyword: Hepatitis B
Published in: Infection / Issue 5/2019
Print ISSN: 0300-8126
Electronic ISSN: 1439-0973
DOI: https://doi.org/10.1007/s15010-019-01306-5

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Analysis of hepatitis B virus-mixed genotype infection by ultra deep pyrosequencing in Sudanese patients, 2015–2016

Abstract

Purpose

Methods

Results

Conclusions

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Abstract

Purpose

Methods

Results

Conclusions

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