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Virology Journal
Published in: Virology Journal 1/2010

Open Access 01-12-2010 | Research

Characterization of Hepatitis B virus (HBV) genotypes in patients from Rondônia, Brazil

Authors: Alcione O Santos, Mónica V Alvarado-Mora, Lívia Botelho, Deusilene S Vieira, João R Rebello Pinho, Flair J Carrilho, Eduardo R Honda, Juan M Salcedo

Published in: Virology Journal | Issue 1/2010

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Abstract

Background

Hepatitis B virus (HBV) can be classified into nine genotypes (A-I) defined by sequence divergence of more than 8% based on the complete genome. This study aims to identify the genotypic distribution of HBV in 40 HBsAg-positive patients from Rondônia, Brazil. A fragment of 1306 bp partially comprising surface and polymerase overlapping genes was amplified by PCR. Amplified DNA was purified and sequenced. Amplified DNA was purified and sequenced on an ABI PRISM® 377 Automatic Sequencer (Applied Biosystems, Foster City, CA, USA). The obtained sequences were aligned with reference sequences obtained from the GenBank using Clustal X software and then edited with Se-Al software. Phylogenetic analyses were conducted by the Markov Chain Monte Carlo (MCMC) approach using BEAST v.1.5.3.

Results

The subgenotypes distribution was A1 (37.1%), D3 (22.8%), F2a (20.0%), D4 (17.1%) and D2 (2.8%).

Conclusions

These results for the first HBV genotypic characterization in Rondônia state are consistent with other studies in Brazil, showing the presence of several HBV genotypes that reflects the mixed origin of the population, involving descendants from Native Americans, Europeans, and Africans.
Appendix
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Metadata
Title
Characterization of Hepatitis B virus (HBV) genotypes in patients from Rondônia, Brazil
Authors
Alcione O Santos
Mónica V Alvarado-Mora
Lívia Botelho
Deusilene S Vieira
João R Rebello Pinho
Flair J Carrilho
Eduardo R Honda
Juan M Salcedo
Publication date
01-12-2010
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2010
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-7-315

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