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Journal of Cancer Research and Clinical Oncology

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Open Access 01-05-2020 | Metastasis | Original Article – Cancer Research

Significance of unphosphorylated and phosphorylated heat shock protein 27 as a prognostic biomarker in pancreatic ductal adenocarcinoma

Authors: Richard Drexler, Kim C. Wagner, Mirco Küchler, Bernd Feyerabend, Moritz Kleine, Karl J. Oldhafer

Published in: Journal of Cancer Research and Clinical Oncology | Issue 5/2020

Abstract

Purpose

Few studies reported about the potential of unphosphorylated heat shock protein 27 (HSP27) and phosphorylated heat shock protein 27 (pHSP27) as a predictor for survival and gemcitabine resistance in pancreatic ductal adenocarcinoma (PDAC). In this study, we analysed the expression patterns of pHSP27 and HSP27 in a patient population after surgery and correlated the immunohistochemical results with clinicopathological data and long-term outcome of the patients.

Methods

HSP27 and pHSP27 (Ser-15, Ser-78 and Ser-82) protein expression were analysed by immunohistochemistry using the immunoreactive score (IRS) from paraffin-embedded tissue of 106 patients with PDAC who underwent surgery. Immunohistochemical results were correlated with clinicopathological data, disease-free (DFS) and overall survival (OS).

Results

HSP27 expression was significantly lower in patients with a shorter OS (p = 0.006) and DFS (p < 0.0001). A higher HSP27 expression was associated with a better response to gemcitabine in the resected, non-metastasised patients group (p = 0.001). Furthermore, HSP27 was downregulated in patients suffering from metastases at time of surgery (p < 0.001) and in undifferentiated tumours (p = 0.007). In contrast, pHSP27-Ser15, -Ser78 and -Ser82 were not associated with any survival data of the study population.

Conclusion

HSP27 seems to be a strong indicator for the prediction of OS and DFS. Moreover, HSP27 could play a role in the formation and migration of liver metastases of PDAC.

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Title: Significance of unphosphorylated and phosphorylated heat shock protein 27 as a prognostic biomarker in pancreatic ductal adenocarcinoma
Authors: Richard Drexler
Kim C. Wagner
Mirco Küchler
Bernd Feyerabend
Moritz Kleine
Karl J. Oldhafer
Publication date: 01-05-2020
Publisher: Springer Berlin Heidelberg
Keywords: Metastasis
Shock
Shock
Biomarkers
Pancreatic Cancer
Pancreatic Cancer
Published in: Journal of Cancer Research and Clinical Oncology / Issue 5/2020
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-020-03175-0

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Significance of unphosphorylated and phosphorylated heat shock protein 27 as a prognostic biomarker in pancreatic ductal adenocarcinoma

Abstract

Purpose

Methods

Results

Conclusion

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Abstract

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