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Open Access 01-06-2021 | Cystitis | Original Article

Effect of irradiation on the expression of E-cadherin and β-catenin in early and late radiation sequelae of the urinary bladder and its modulation by NF-κB inhibitor thalidomide

Authors: Alexander Krischak, Jakob Kowaliuk, Sina Sarsarshahi, Wolfgang Dörr, Ao.Univ.-Prof. Dr. med.vet. Dipl.ACVR-RO Dipl.ECVIM-CA (Oncol) Miriam Kleiter

Published in: Strahlentherapie und Onkologie | Issue 6/2021

Abstract

Purpose

In a previous study we have shown in a mouse model that administration of nuclear factor-kappa B (NF-κB) inhibitor thalidomide has promising therapeutic effects on early radiation cystitis (ERC) and late radiation sequelae (LRS) of the urinary bladder. The aim of this study was to evaluate in the same mice the effect of thalidomide on adherens junction (AJ) proteins in ERC and LRS.

Methods

Urothelial expressions of E‑cadherin and β‑catenin were assessed by immunohistochemistry in formalin-fixed paraffin-embedded (FFPE) bladder specimens over 360 days post single-dose irradiation on day 0. First, the effect of irradiation on AJ expression and then effects of thalidomide on irradiation-induced AJ alterations were assessed using three different treatment times.

Results

Irradiation provoked a biphasic upregulation of E‑cadherin and β‑catenin in the early phase. After a mild decrease of E‑cadherin and a pronounced decrease of β‑catenin at the end of the early phase, both increased again in the late phase. Early administration of thalidomide (day 1–15) resulted in a steeper rise in the first days, an extended and increased expression at the end of the early phase and a higher expression of β‑catenin alone at the beginning of the late phase.

Conclusion

Upregulation of AJ proteins is an attempt to compensate irradiation-induced impairment of urothelial barrier function. Early administration of thalidomide improves these compensatory mechanisms by inhibiting NF-κB signaling and its interfering effects.

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Title: Effect of irradiation on the expression of E-cadherin and β-catenin in early and late radiation sequelae of the urinary bladder and its modulation by NF-κB inhibitor thalidomide
Authors: Alexander Krischak
Jakob Kowaliuk
Sina Sarsarshahi
Wolfgang Dörr
Ao.Univ.-Prof. Dr. med.vet. Dipl.ACVR-RO Dipl.ECVIM-CA (Oncol) Miriam Kleiter
Publication date: 01-06-2021
Publisher: Springer Berlin Heidelberg
Keywords: Cystitis
Radiotherapy
Cystitis
Published in: Strahlentherapie und Onkologie / Issue 6/2021
Print ISSN: 0179-7158
Electronic ISSN: 1439-099X
DOI: https://doi.org/10.1007/s00066-021-01751-y

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Effect of irradiation on the expression of E-cadherin and β-catenin in early and late radiation sequelae of the urinary bladder and its modulation by NF-κB inhibitor thalidomide

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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