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Published in: BMC Infectious Diseases 1/2020

Open Access 01-12-2020 | Leishmania | Research article

Frequency of Leishmania spp. infection among HIV-infected patients living in an urban area in Brazil: a cross-sectional study

Authors: M. A. Cunha, B. J. Celeste, N. Kesper, M. Fugimori, M. M. Lago, A. S. Ibanes, L. M. Ouki, E. A. Simões Neto, F. F. Fonseca, M. A. L. Silva, W. L. Barbosa Júnior, J. A. L. Lindoso

Published in: BMC Infectious Diseases | Issue 1/2020

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Abstract

Background

There is little information about the frequency of Leishmania infection in asymptomatic people living with HIV (PLWH) and about the performance of laboratory diagnostic methods in coinfected patients in Latin America. The main objective of this study is to evaluate the frequency of Leishmania spp. infection in HIV-infected patients living in an urban area in Brazil.

Methods

To detect Leishmania infection, diagnostic tests were performed to detect anti-Leishmania antibodies (ELISA using Leptomonas seymouri antigens; ELISA using rK39 antigens; ELISA using rK28 antigens; indirect fluorescent-antibody test (IFAT); direct agglutination test (DAT)) and Leishmania DNA (polymerase chain reaction (PCR) with the target genes kDNA and ITS-1).

Results

The frequency of at least one positive test was 15%. For ELISA using Leptomonas antigens and IFAT, there was an association between CD4+ T lymphocyte counts and test positivity, with a higher positivity of these tests in more immunosuppressed patients (CD4+ T cell count < 200/mm3).

Conclusions

According to our data, there was a high prevalence of Leishmania spp. infections in this population living with HIV. Although there is the possibility of cross-reaction, some tests that are considered highly specific for the diagnosis of Leishmania infection were positive. There was also an association between the positivity of some tests studied and lower values of CD4+ T lymphocytes.
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Metadata
Title
Frequency of Leishmania spp. infection among HIV-infected patients living in an urban area in Brazil: a cross-sectional study
Authors
M. A. Cunha
B. J. Celeste
N. Kesper
M. Fugimori
M. M. Lago
A. S. Ibanes
L. M. Ouki
E. A. Simões Neto
F. F. Fonseca
M. A. L. Silva
W. L. Barbosa Júnior
J. A. L. Lindoso
Publication date
01-12-2020
Publisher
BioMed Central
Published in
BMC Infectious Diseases / Issue 1/2020
Electronic ISSN: 1471-2334
DOI
https://doi.org/10.1186/s12879-020-05622-2

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