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01-12-2020 | Ulcerative Colitis | Original Article

Jasonia glutinosa (L.) DC., a traditional herbal medicine, reduces inflammation, oxidative stress and protects the intestinal barrier in a murine model of colitis

Authors: Marta Sofía Valero, Mateo González, Mariano Ramón-Gimenez, Paula B. Andrade, Eduardo Moreo, Francisco Les, Fátima Fernandes, Carlota Gómez-Rincón, César Berzosa, José Antonio García de Jalón, Mª. Pilar Arruebo, Miguel Ángel Plaza, Ralf Köhler, Víctor López, Patricia Valentão, Marta Castro

Published in: Inflammopharmacology | Issue 6/2020

Abstract

Jasonia glutinosa (L.) DC., known as rock tea (RT), is traditionally used in Spain as a digestive due to its beneficial properties in bowel disorders. The pharmacological nature of these properties has not been established yet. The aim of this work was to evaluate the therapeutic utility of RT in experimental colitis and to identify chemical constituents with anti-inflammatory and/or anti-oxidative properties. RT extract was prepared with ethanol in a Soxhlet apparatus and analysed by HPLC–DAD. Superoxide radical scavenging properties, xanthine oxidase and lipoxygenase (5-LOX) inhibitory activity, and capability to lower nitric oxide (NO) and tumor necrosis factor α (TNF-α) levels were measured in cell-free and cell-based assays. In the 2.5%-dextran-sodium sulphate (DSS) injury-repair model of ulcerative colitis (UC), mice were daily treated with sulfasalazine (SSZ, as reference drug, 100 mg/kg bw), RT (5, 25 and 50 mg/kg bw, p.o.), or vehicle over 20 days. Colitis was scored daily. Colon samples were examined macroscopically and histopathologically. Protein levels of myeloperoxidase (MPO), interleukins 6, and 10 (IL-6, IL-10), inducible NO synthase (iNOS), and cyclooxygenase-2 (COX-2) were studied as markers of oxidative stress and inflammatory activity. The integrity of the apical epithelial layer was assessed by immunofluorescence staining of zonula ocludens-1 (ZO-1). Finally, intestinal contractility was also evaluated by isometric myography. Fifteen phenolic compounds and three pigments were identified and quantified, of which caffeoylquinic acids, and the flavonoid, quercetin-3-O-galactoside, were the most abundant. RT extract significantly scavenged superoxide radicals, inhibited 5-LOX activity, and lowered NO and TNF-α levels. DSS-treated mice receiving RT scored clinically lower than controls during the first 3 days of DSS treatment and during the recovery period. SSZ was less effective than RT. Anatomical and histological examination of colon samples revealed that RT significantly prevented colon shortening, increased colon thickness, and lowered the macroscopic damage score. RT also significantly prevented the increase of MPO activity, IL-6 levels, iNOS and COX-2 expression, the loss of ZO-1 apical expression, and normalized contractility disturbances. In conclusion, daily administration of RT showed therapeutic properties in the DSS-model of UC. The benefits of RT can likely be attributed to its anti-inflammatory and antioxidant phenolic and flavonoid constituents.

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Title: Jasonia glutinosa (L.) DC., a traditional herbal medicine, reduces inflammation, oxidative stress and protects the intestinal barrier in a murine model of colitis
Authors: Marta Sofía Valero
Mateo González
Mariano Ramón-Gimenez
Paula B. Andrade
Eduardo Moreo
Francisco Les
Fátima Fernandes
Carlota Gómez-Rincón
César Berzosa
José Antonio García de Jalón
Mª. Pilar Arruebo
Miguel Ángel Plaza
Ralf Köhler
Víctor López
Patricia Valentão
Marta Castro
Publication date: 01-12-2020
Publisher: Springer International Publishing
Keywords: Ulcerative Colitis
Xanthine Alkaloid
Sulfasalazine
Published in: Inflammopharmacology / Issue 6/2020
Print ISSN: 0925-4692
Electronic ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-019-00626-0

At a glance: The STEP trials

Springer Medicine

Jasonia glutinosa (L.) DC., a traditional herbal medicine, reduces inflammation, oxidative stress and protects the intestinal barrier in a murine model of colitis

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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