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Open Access 01-12-2020 | Ulcerative Colitis | Original Article

Paeoniflorin ameliorates ulcerative colitis by modulating the dendritic cell-mediated T_H17/T_reg balance

Authors: Kai Zheng, Jia Jia, Shihai Yan, Hong Shen, Ping Zhu, Jiangyi Yu

Published in: Inflammopharmacology | Issue 6/2020

Abstract

Immunological tolerance is critical for maintaining gut homeostasis. An imbalance between interleukin-17 (IL-17)-producing T helper 17 (T_H17) cells and regulatory T cells (T_reg cells) is involved in ulcerative colitis (UC) pathogenesis. Dendritic cells (DCs) are able to induce T cell differentiation. Paeoniflorin (PF) is a monoterpene glucoside that is commonly used for treatment of autoimmune disease. However, the immunological mechanism of PF involvement in UC treatment is unclear. The present study aimed to explore whether PF can restore the T_H17/T_reg balance by modulating DCs. The effects of PF on DCs, T_H17 cells and T_reg cells were measured. Furthermore, PF-treated DCs were injected into mice with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. PF inhibited MHC-II and CD86 expression on the DC surface (P < 0.05), decreased interleukin (IL)-12 secretion in vitro and in vivo (P < 0.05), and restored the T_H17/T_reg ratio in the mouse model of colitis (P < 0.05). PF-treated DCs diminished T_H17 differentiation (4.26% in vitro and 1.64% in vivo) and decreased IL-17 expression (P < 0.05) while inducing CD4⁺CD25⁺Foxp3⁺ T_reg differentiation (7.82% in vitro and 6.85% in vivo) and increasing Foxp3 and IL-10 production (P < 0.05). Additionally, both PF and PF-treated DCs improved colonic histopathology in the mouse model of colitis (P < 0.05). In conclusion this study suggested that PF can ameliorate TNBS-induced colitis by modulating the DC-mediated T_H17/T_reg balance.

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Title: Paeoniflorin ameliorates ulcerative colitis by modulating the dendritic cell-mediated TH17/Treg balance
Authors: Kai Zheng
Jia Jia
Shihai Yan
Hong Shen
Ping Zhu
Jiangyi Yu
Publication date: 01-12-2020
Publisher: Springer International Publishing
Keyword: Ulcerative Colitis
Published in: Inflammopharmacology / Issue 6/2020
Print ISSN: 0925-4692
Electronic ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-020-00722-6

2024 ASCO Annual Meeting

Springer Medicine

Paeoniflorin ameliorates ulcerative colitis by modulating the dendritic cell-mediated T_H17/T_reg balance

Abstract

2024 ASCO Annual Meeting

Springer Medicine

Abstract

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