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Cancer Cell International

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Open Access 01-12-2020 | Thyroid Cancer | Primary research

TMPO-AS1 promotes cell proliferation of thyroid cancer via sponging miR-498 to modulate TMPO

Authors: Zhenyu Li, Yun Feng, Zhen Zhang, Xiaozhong Cao, Xiubo Lu

Published in: Cancer Cell International | Issue 1/2020

Abstract

Background

Thyroid cancer (TC) is the most frequent endocrine malignancy. Long noncoding RNAs (lncRNAs) have been confirmed to act as significant roles in tumor development. The role of lncRNA TMPO-AS1 in TC is still unclear, so it remains to be explored. The aim of the research is to investigate the role and regulatory mechanism of TMPO-AS1 in TC.

Methods

TMPO-AS1 and TMPO expression in TC tumors and cells was detected by TCGA database and QRT-PCR assay respectively. CCK-8, EDU, TUNEL and western blot assays were conducted to identify the biological functions of TMPO-AS1 in TC. Luciferase reporter and RNA pull down assays were conducted to measure the interaction among TMPO-AS1, TMPO and miR-498.

Results

TMPO-AS1 was overexpressed in TC tissues and cell lines. Knockdown of TMPO-AS1 suppressed cell growth and accelerated cell apoptosis in TC. Furthermore, downregulation of TMPO-AS1 suppressed TMPO expression in TC. The data suggested that TMPO expression was upregulated in TC tissues and cell lines and was positively correlated with TMPO-AS1 expression in TC. Furthermore, the expression of miR-498 presented low expression in TC cells. And miR-498 expression was negatively regulated by TMPO-AS1, meanwhile, TMPO expression was negatively regulated by miR-498 in TC cells. Besides, it was confirmed that TMPO-AS1 could bind with miR-498 and TMPO in TC cells. In addition, it was validated that TMPO-AS1 elevated the levels of TMPO via sponging miR-498 in TC cells.

Conclusions

TMPO-AS1 promotes cell proliferation in TC via sponging miR-498 to modulate TMPO.

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Title: TMPO-AS1 promotes cell proliferation of thyroid cancer via sponging miR-498 to modulate TMPO
Authors: Zhenyu Li
Yun Feng
Zhen Zhang
Xiaozhong Cao
Xiubo Lu
Publication date: 01-12-2020
Publisher: BioMed Central
Keywords: Thyroid Cancer
Thyroid Cancer
Thyroid Cancer
Published in: Cancer Cell International / Issue 1/2020
Electronic ISSN: 1475-2867
DOI: https://doi.org/10.1186/s12935-020-01334-4

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