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BMC Oral Health
Published in: BMC Oral Health 1/2023

Open Access 01-12-2023 | Stem Cell Transplantation | Research

The salivary proteome in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients: a labelled and label-free proteomics approach

Authors: S. J. M. van Leeuwen, G. B. Proctor, A. Staes, A. M. G. A. Laheij, C. M. J. Potting, M. T. Brennan, I. von Bültzingslöwen, F. R. Rozema, M. D. Hazenberg, N. M. A. Blijlevens, J. E. Raber-Durlacher, M. C. D. N. J. M. Huysmans

Published in: BMC Oral Health | Issue 1/2023

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Abstract

Background

Oral mucositis is a frequently seen complication in the first weeks after hematopoietic stem cell transplantation recipients which can severely affects patients quality of life. In this study, a labelled and label-free proteomics approach were used to identify differences between the salivary proteomes of autologous hematopoietic stem cell transplantation (ASCT) recipients developing ulcerative oral mucositis (ULC-OM; WHO score ≥ 2) or not (NON-OM).

Methods

In the TMT-labelled analysis we pooled saliva samples from 5 ULC-OM patients at each of 5 timepoints: baseline, 1, 2, 3 weeks and 3 months after ASCT and compared these with pooled samples from 5 NON-OM patients. For the label-free analysis we analyzed saliva samples from 9 ULC-OM and 10 NON-OM patients at 6 different timepoints (including 12 months after ASCT) with Data-Independent Acquisition (DIA). As spectral library, all samples were grouped (ULC-OM vs NON-OM) and analyzed with Data Dependent Analysis (DDA). PCA plots and a volcano plot were generated in RStudio and differently regulated proteins were analyzed using GO analysis with g:Profiler.

Results

A different clustering of ULC-OM pools was found at baseline, weeks 2 and 3 after ASCT with TMT-labelled analysis. Using label-free analysis, week 1–3 samples clustered distinctly from the other timepoints. Unique and up-regulated proteins in the NON-OM group (DDA analysis) were involved in immune system-related processes, while those proteins in the ULC-OM group were intracellular proteins indicating cell lysis.

Conclusions

The salivary proteome in ASCT recipients has a tissue protective or tissue-damage signature, that corresponded with the absence or presence of ulcerative oral mucositis, respectively.

Trial registration

The study is registered in the national trial register (NTR5760; automatically added to the International Clinical Trial Registry Platform).
Appendix
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Metadata
Title
The salivary proteome in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients: a labelled and label-free proteomics approach
Authors
S. J. M. van Leeuwen
G. B. Proctor
A. Staes
A. M. G. A. Laheij
C. M. J. Potting
M. T. Brennan
I. von Bültzingslöwen
F. R. Rozema
M. D. Hazenberg
N. M. A. Blijlevens
J. E. Raber-Durlacher
M. C. D. N. J. M. Huysmans
Publication date
01-12-2023
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2023
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-023-03190-w

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