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Clinical and Translational Medicine
Published in: Clinical and Translational Medicine 1/2017

Open Access 01-12-2017 | Review

Single-cell sequencing and tumorigenesis: improved understanding of tumor evolution and metastasis

Authors: Darrell L. Ellsworth, Heather L. Blackburn, Craig D. Shriver, Shahrooz Rabizadeh, Patrick Soon-Shiong, Rachel E. Ellsworth

Published in: Clinical and Translational Medicine | Issue 1/2017

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Abstract

Extensive genomic and transcriptomic heterogeneity in human cancer often negatively impacts treatment efficacy and survival, thus posing a significant ongoing challenge for modern treatment regimens. State-of-the-art DNA- and RNA-sequencing methods now provide high-resolution genomic and gene expression portraits of individual cells, facilitating the study of complex molecular heterogeneity in cancer. Important developments in single-cell sequencing (SCS) technologies over the past 5 years provide numerous advantages over traditional sequencing methods for understanding the complexity of carcinogenesis, but significant hurdles must be overcome before SCS can be clinically useful. In this review, we: (1) highlight current methodologies and recent technological advances for isolating single cells, single-cell whole-genome and whole-transcriptome amplification using minute amounts of nucleic acids, and SCS, (2) summarize research investigating molecular heterogeneity at the genomic and transcriptomic levels and how this heterogeneity affects clonal evolution and metastasis, and (3) discuss the promise for integrating SCS in the clinical care arena for improved patient care.
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Metadata
Title
Single-cell sequencing and tumorigenesis: improved understanding of tumor evolution and metastasis
Authors
Darrell L. Ellsworth
Heather L. Blackburn
Craig D. Shriver
Shahrooz Rabizadeh
Patrick Soon-Shiong
Rachel E. Ellsworth
Publication date
01-12-2017
Publisher
Springer Berlin Heidelberg
Published in
Clinical and Translational Medicine / Issue 1/2017
Electronic ISSN: 2001-1326
DOI
https://doi.org/10.1186/s40169-017-0145-6

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