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01-07-2012 | Translational Research and Biomarkers

Silencing of Glucose-Regulated Protein 78 (GRP78) Enhances Cell Migration Through the Upregulation of Vimentin in Hepatocellular Carcinoma Cells

Authors: Po-Li Wei, MD, PhD, Li-Jen Kuo, MD, Weu Wang, MD, Feng-Yen Lin, PhD, Hui-Hsiung Liu, MD, PhD, Tseng How, PhD, Yuan-Soon Ho, PhD, Ming-Te Huang, MD, Chih-Hsiung Wu, MD, PhD, Yu-Jia Chang, PhD

Published in: Annals of Surgical Oncology | Special Issue 3/2012

Abstract

Background

Glucose-regulated protein 78 (GRP78) plays an important role in embryonic development and cancer progression. However, there is little information regarding the regulation of GRP78 in hepatocellular carcinoma (HCC) metastasis.

Methods

We used RNA silencing and cDNA expression vectors to manipulate target gene expression in HCC cells. The transwell migration assay and xCelligence biosensor system were applied to determine the proliferatory and migratory ability of the HCC cells.

Results

In this study, we found that GRP78 silencing enhanced cell migration in both HepJ5 and Mahlavu cells. Overexpressed GRP78 in skHep1 cells suppressed the migratory ability. In the insight mechanism dissection for GRP78-mediated cancer migration, we found that downregulation of GRP78 caused the increase of vimentin expression on HCC cells. Suppressed vimentin expression also decreased the migratory ability on HCC, indicating that vimentin expression levels modulated the cell migratory ability.

Conclusion

We found that silencing GRP78 in HCC cells may enhance cell migration through the increase of vimentin expression.

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Title: Silencing of Glucose-Regulated Protein 78 (GRP78) Enhances Cell Migration Through the Upregulation of Vimentin in Hepatocellular Carcinoma Cells
Authors: Po-Li Wei, MD, PhD
Li-Jen Kuo, MD
Weu Wang, MD
Feng-Yen Lin, PhD
Hui-Hsiung Liu, MD, PhD
Tseng How, PhD
Yuan-Soon Ho, PhD
Ming-Te Huang, MD
Chih-Hsiung Wu, MD, PhD
Yu-Jia Chang, PhD
Publication date: 01-07-2012
Publisher: Springer-Verlag
Published in: Annals of Surgical Oncology / Issue Special Issue 3/2012
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI: https://doi.org/10.1245/s10434-011-2055-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Silencing of Glucose-Regulated Protein 78 (GRP78) Enhances Cell Migration Through the Upregulation of Vimentin in Hepatocellular Carcinoma Cells

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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