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The journal of nutrition, health & aging
Published in: The journal of nutrition, health & aging 5/2016

01-05-2016

Resveratrol requires red wine polyphenols for optimum antioxidant activity

Published in: The journal of nutrition, health & aging | Issue 5/2016

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Abstract

Objective

There is substantial evidence that a diet rich in fruit and vegetables may reduce the risk of aging and stress oxidative associated diseases. It has been suggested that benefits associated with fruit and red wine consumption could be due to pooled antioxidant microcomponents in diet. The aim of this study was to investigate the antioxidant activities of pure resveratrol (a well known phytoalexin, RSV) and red wine polyphenols (RWP), using UV-B radiated isolated rat hepatocytes as a model of oxidative stress.

Methods

Rat hepatocytes were isolated by the collagenase method. The cells were loaded with resveratrol and/or polyphenols at different concentrations. The production of thiobarbituric acid reactive substances (TBARS) released by UV-B radiated cells and the levels of lipid-soluble antioxidants (Dolichol, Vitamin E, Coenzyme Q9 and Q10) were measured.

Results

Resveratrol had pro-oxidant or antioxidant effects depending on (lower or higher) dosage. RWP protection from photolipoperoxidation was dose-dependent and increased with dosage. Combination of the two compounds exhibited synergistic antioxidant effect, and made resveratrol effective both at lower and higher dosages.

Conclusions

These results suggest that resveratrol requires red wine polyphenols for optimum antioxidant activity.
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Metadata
Title
Resveratrol requires red wine polyphenols for optimum antioxidant activity
Publication date
01-05-2016
Published in
The journal of nutrition, health & aging / Issue 5/2016
Print ISSN: 1279-7707
Electronic ISSN: 1760-4788
DOI
https://doi.org/10.1007/s12603-015-0611-z

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