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Obesity Surgery

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22-02-2024 | Respiratory Microbiota | Original Contributions

Analysis of 16s rRNA Gene Sequencing in Feces: The Impact of Bariatric Surgery on the Gut Microbiota in Patients with Obesity

Authors: Lijun Zhang, Xiaolei Cheng, Lianli Xia, Na Liu, Liyin Liu, Shujuan Liu, Shaojia Wang, Jianhui Yin

Published in: Obesity Surgery | Issue 4/2024

Abstract

Purpose

Obesity is a risk factor for many chronic diseases. This study aimed to investigate the effect of bariatric surgery on the gut microbiota from patients with obesity.

Materials and Methods

The microbiota composition from stool samples before and after bariatric surgery were identified using bacterial 16S rRNA gene sequencing. Based on the speed of weight loss, patients were classified as the slow-loss group and fast-loss group. The ɑ- and β-diversity analysis was done to compare the species richness, evenness, and overall structure of the microbiota between different groups. Next, linear discriminant analysis effect size (LEfSe) and receiver operating characteristic (ROC) analysis were implemented to identify high-dimensional biomarkers and significantly different species of microbial taxa between different groups. Finally, the pathway analysis was inferred using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) to predict the functional profiling of microbial communities.

Results

β-diversity analysis suggested that species diversity of preoperative samples of slow-loss group was significantly higher than the fast-loss group. High levels of Oscillospira and Abiotrophia in the preoperative gut microbiota may lead to poor postoperative weight loss. For patients with poor postoperative weight loss due to changes in gut microbiota, the gut microbiota is mainly composed of Lactobacillus. For patients with good postoperative results, the gut microbiota is mainly composed of Escherichia, Robinsonella, and Dialister. In addition, multiple metabolic-related pathways were significantly different between the four groups.

Conclusion

This comparative study revealed biomarker species based on microfloral composition in patients with obesity before and after bariatric surgery.

Graphical Abstract

Available only for authorised users

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Title: Analysis of 16s rRNA Gene Sequencing in Feces: The Impact of Bariatric Surgery on the Gut Microbiota in Patients with Obesity
Authors: Lijun Zhang
Xiaolei Cheng
Lianli Xia
Na Liu
Liyin Liu
Shujuan Liu
Shaojia Wang
Jianhui Yin
Publication date: 22-02-2024
Publisher: Springer US
Keywords: Respiratory Microbiota
Obesity
Obesity
Bariatric Surgery
Bariatric Surgery
Biomarkers
Escherichia Coli
Published in: Obesity Surgery / Issue 4/2024
Print ISSN: 0960-8923
Electronic ISSN: 1708-0428
DOI: https://doi.org/10.1007/s11695-024-07087-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Analysis of 16s rRNA Gene Sequencing in Feces: The Impact of Bariatric Surgery on the Gut Microbiota in Patients with Obesity

Abstract

Purpose

Materials and Methods

Results

Conclusion

Graphical Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Materials and Methods

Results

Conclusion

Graphical Abstract

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