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Molecular Cancer
Published in: Molecular Cancer 1/2017

Open Access 01-12-2017 | Letter to the Editor

Profiling, clinicopathological correlation and functional validation of specific long non-coding RNAs for hepatocellular carcinoma

Authors: Jian Yao, Lingjiao Wu, Xiaohua Meng, Huanxia Yang, Shujun Ni, Qiangfeng Wang, Jiawei Zhou, Qiong Zhang, Kunkai Su, Li Shao, Qingyi Cao, Mingding Li, Fusheng Wu, Lanjuan Li

Published in: Molecular Cancer | Issue 1/2017

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Abstract

Background

Hepatocellular carcinoma (HCC) is one of the most prevalent and aggressive malignancies worldwide. Studies seeking to advance the overall understanding of lncRNA profiling in HCC remain rare.

Methods

The transcriptomic profiling of 12 HCC tissues and paired adjacent normal tissues was determined using high-throughput RNA sequencing. Fifty differentially expressed mRNAs (DEGs) and lncRNAs (DELs) were validated in 21 paired HCC tissues via quantitative real-time PCR. The correlation between the expression of DELs and various clinicopathological characteristics was analyzed using Student’s t-test or linear regression. Co-expression networks between DEGs and DELs were constructed through Pearson correlation co-efficient and enrichment analysis. Validation of DELs’ functions including proliferation and migration was performed via loss-of-function RNAi assays.

Results

In this study, we identified 439 DEGs and 214 DELs, respectively, in HCC. Furthermore, we revealed that multiple DELs, including NONHSAT003823, NONHSAT056213, NONHSAT015386 and especially NONHSAT122051, were remarkably correlated with tumor cell differentiation, portal vein tumor thrombosis, and serum or tissue alpha fetoprotein levels. In addition, the co-expression network analysis between DEGs and DELs showed that DELs were involved with metabolic, cell cycle, chemical carcinogenesis, and complement and coagulation cascade-related pathways. The silencing of the endogenous level of NONHSAT122051 or NONHSAT003826 could significantly attenuate the mobility of both SK-HEP-1 and SMMC-7721 HCC cells.

Conclusion

These findings not only add knowledge to the understanding of genome-wide transcriptional evaluation of HCC but also provide promising targets for the future diagnosis and treatment of HCC.
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Metadata
Title
Profiling, clinicopathological correlation and functional validation of specific long non-coding RNAs for hepatocellular carcinoma
Authors
Jian Yao
Lingjiao Wu
Xiaohua Meng
Huanxia Yang
Shujun Ni
Qiangfeng Wang
Jiawei Zhou
Qiong Zhang
Kunkai Su
Li Shao
Qingyi Cao
Mingding Li
Fusheng Wu
Lanjuan Li
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Molecular Cancer / Issue 1/2017
Electronic ISSN: 1476-4598
DOI
https://doi.org/10.1186/s12943-017-0733-5

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