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Cancer Cell International
Published in: Cancer Cell International 1/2020

01-12-2020 | osteosarcoma | Primary research

The novel circ_0028171/miR-218-5p/IKBKB axis promotes osteosarcoma cancer progression

Authors: Feng Pan, Jun Zhang, Benseng Tang, Li Jing, Bing Qiu, Zhengang Zha

Published in: Cancer Cell International | Issue 1/2020

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Abstract

Background

Recently, it has been demonstrated that circular RNA (circRNA) contributes to the production and progression in human cancer. However, the specific function and underlying mechanism of circ_0028171 in osteosarcoma (OS) still remain largely unclear and require to be investigated.

Methods

In our study, we confirmed differentially expressed circRNAs by microarray analysis in normal bone cells vs. OS cell lines. The expression of circ-0028171 in OS was measured by qRT-PCR. Nuclear-cytoplasmic fractionation was employed to identify the localization of circ-0028171, and RNase R and actinomycin D treatment were used to prove its circular characteristic. In vitro experiments, such as CCK-8 method, cell count, cell colony formation, transwell migration and invasion assays, and in vivo tumor models were adopted to evaluate the effect of circ_0028171. Further, luciferase reporter, RIP and RNA pull-down assays were conducted to confirm the binding sites of circ_0028171 with miR-218-5p.

Results

We found that circ_0028171 displayed a remarkably higher expression in both OS tissues and cell lines. Circ_0028171 mainly located in the cytoplasm as a stable cyclic transcript. Knockdown of circ_0028171 suppressed OS tumor growth in vitro and in vivo, while up-regulated circ_0028171 remarkably enhanced cell proliferation, migration and invasion abilities in OS. Several mechanistic experiments revealed that circ_0028171 served as a sponge of miR-218-5p to increase IKBKB expression.

Conclusions

our research reveals that circ_0028171 might promote the malignant behavior of OS tissues through miR-218-5p/IKBKB axis, which could be a potential novel marker for early diagnosis of OS.
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Metadata
Title
The novel circ_0028171/miR-218-5p/IKBKB axis promotes osteosarcoma cancer progression
Authors
Feng Pan
Jun Zhang
Benseng Tang
Li Jing
Bing Qiu
Zhengang Zha
Publication date
01-12-2020
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2020
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-020-01562-8

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