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Archives of Virology
Published in: Archives of Virology 11/2015

01-11-2015 | Original Article

Molecular epidemiology of porcine reproductive and respiratory syndrome viruses isolated from 1991 to 2013 in Taiwan

Authors: Ming-Chung Deng, Chia-Yi Chang, Tien-Shine Huang, Hsiang-Jung Tsai, Chieh Chang, Fun-In Wang, Yu-Liang Huang

Published in: Archives of Virology | Issue 11/2015

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Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) was first identified in Taiwan in 1991, but the genetic diversity and evolution of PRRSV has not been thoroughly investigated over the past 20 years. The aim of this study was to bridge the gap in understanding of its molecular epidemiology. A total of 31 PRRSV strains were collected and sequenced. The sequences were aligned using the MUSCLE program, and phylogenetic analysis were performed by the maximum-likelihood method and the neighbor-joining method using MEGA 5.2 software. In the early 1990s, two prototype strains, WSV and MD001 of the North American genotype, were first identified. Over the years, both viruses evolved separately. The population dynamics of PRRSV revealed that the strains of the MD001 group were predominant in Taiwan. Evolution was manifested in changes in the nsp2 and ORF5 genes. In addition, a suspected newly invading exotic strain was recovered in 2013, suggesting that international spread is still taking place and that it is affecting the population dynamics. Overall, the results provide an important basis for vaccine development for the control and prevention of PRRS.
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Metadata
Title
Molecular epidemiology of porcine reproductive and respiratory syndrome viruses isolated from 1991 to 2013 in Taiwan
Authors
Ming-Chung Deng
Chia-Yi Chang
Tien-Shine Huang
Hsiang-Jung Tsai
Chieh Chang
Fun-In Wang
Yu-Liang Huang
Publication date
01-11-2015
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 11/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-015-2554-4

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