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Open Access 01-12-2022 | Migraine | Research

Leukocyte inflammatory phenotype and function in migraine patients compared with matched non-migraine volunteers: a pilot study

Authors: Hongtao Li, Qiang Fu, Kamaira Philips, Yufei Sun, Keturah R. Faurot, Susan A. Gaylord, John Douglas Mann

Published in: BMC Neurology | Issue 1/2022

Abstract

Background

Migraine is a neurological condition characterized by chronic inflammation. However, not much is known about the potential role of peripheral blood immune cells in the pathophysiology of migraine.

Methods

We investigated the status of peripheral blood immune cells of 15 adults with frequent episodic or chronic migraine recruited chronologically from a randomized clinical trial (RCT) on Nutrition for Migraine (NCCIH 5R01AT007813-05) and 15 non-migraine, healthy volunteers (control) matched by age, gender, and Body Mass Index (BMI).

Continuous variables were presented as means ± standard deviationas well as medians, and comparisons between patients and healthy volunteers were performed with non-parametric Wilcoxon signed rank tests. Statistical analysis was performed using Stata (StataCorp. 2019. Stata Statistical Software). Fluorescence-Activated Cell Sorting (FACS) data were processed using FlowJo software (Ashland, OR: Becton, Dickenson and Company; 2019).

Results

We observed that migraineurs had a significantly lower percentage of non-classical monocytes (CD14⁺CD16⁺⁺) in blood circulation, compared to the control group. In addition, Migraineurs also showed a significantly lower percentage of blood CD3⁺CD4⁺ helper T cells and CD4⁺CD25⁺ regulatory T cells, compared to controls. Differences in leukocyte surface markers between chronic migraine patients and their matched controls were more prominent than those between episodic migraine patients and their matched controls.

Conclusions

Our results suggest that migraine is associated with dysregulated peripheral immune homeostasis and that inflammation and autoimmunity may play a role in its pathophysiology.

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Title: Leukocyte inflammatory phenotype and function in migraine patients compared with matched non-migraine volunteers: a pilot study
Authors: Hongtao Li
Qiang Fu
Kamaira Philips
Yufei Sun
Keturah R. Faurot
Susan A. Gaylord
John Douglas Mann
Publication date: 01-12-2022
Publisher: BioMed Central
Keyword: Migraine
Published in: BMC Neurology / Issue 1/2022
Electronic ISSN: 1471-2377
DOI: https://doi.org/10.1186/s12883-022-02781-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Leukocyte inflammatory phenotype and function in migraine patients compared with matched non-migraine volunteers: a pilot study

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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