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Journal of Orthopaedic Surgery and Research

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Open Access 01-12-2019 | Bone Defect | Research article

Autologous cell-coated particles for the treatment of segmental bone defects—a new cell therapy approach

Authors: Dror Ben-David, Bettina Fishman, Guy Rubin, Atara Novak, Ilana Laevsky, Avinoam Kadouri, Yasmin Nishri Katz, Ora Burger, Asaph Zaretsky, Noam Bor, Ephraim Tzur, Shai Meretzki, Nimrod Rozen

Published in: Journal of Orthopaedic Surgery and Research | Issue 1/2019

Abstract

Background

Adipose tissue-derived mesenchymal stem cells (AT-MSCs) are one of the most potent adult stem cells, capable of differentiating into bone, cartilage, adipose, muscle, and others. An innovative autologous AT-MSC-derived cell-based product (BonoFill-II) for bone tissue regeneration was developed to be suited as a bone graft for segmental bone defects.

Methods

BonoFill-II was transplanted into 8 sheep with 3.2-cm full cortex segmental defect formed in the tibia. Bone regeneration was followed by X-ray radiographs for 12 weeks. At experiment termination, the healed tibia bones were analyzed by computed tomography, histology, and mechanical tests.

Results

Our results indicate that one dose of BonoFill-II injectable formula led to an extensive bone growth within the transplantation site and to a complete closure of the critical gap in the sheep’s tibia in a relatively short time (8–12 weeks), with no inflammation and no other signs of graft rejection. This new and innovative product opens new prospects for the treatment of long bone defects.

Conclusions

Injection of BonoFill-II (an innovative autologous cell therapy product for bone tissue regeneration) into a critical size segmental defect model (3.2 cm), generated in the sheep tibia, achieved full bridging of the gap in an extremely short period (8–12 weeks).

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Title: Autologous cell-coated particles for the treatment of segmental bone defects—a new cell therapy approach
Authors: Dror Ben-David
Bettina Fishman
Guy Rubin
Atara Novak
Ilana Laevsky
Avinoam Kadouri
Yasmin Nishri Katz
Ora Burger
Asaph Zaretsky
Noam Bor
Ephraim Tzur
Shai Meretzki
Nimrod Rozen
Publication date: 01-12-2019
Publisher: BioMed Central
Keyword: Bone Defect
Published in: Journal of Orthopaedic Surgery and Research / Issue 1/2019
Electronic ISSN: 1749-799X
DOI: https://doi.org/10.1186/s13018-019-1219-5

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Springer Medicine

Autologous cell-coated particles for the treatment of segmental bone defects—a new cell therapy approach

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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