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Virology Journal
Published in: Virology Journal 1/2022

Open Access 01-12-2022 | Influenza Virus | Methodology

Reverse transcription recombinase-aided amplification assay for H5 subtype avian influenza virus

Authors: Suchun Wang, Yang Li, Fuyou Zhang, Nan Jiang, Qingye Zhuang, Guangyu Hou, Lijian Jiang, Jianmin Yu, Xiaohui Yu, Hualei Liu, Chenglong Zhao, Liping Yuan, Baoxu Huang, Kaicheng Wang

Published in: Virology Journal | Issue 1/2022

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Abstract

Background

The H5 subtype avian influenza virus (AIV) has caused huge economic losses to the poultry industry and is a threat to human health. A rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks.

Methods

In this study, we developed a reverse transcription recombinase-aided amplification (RT-RAA) assay for the detection of H5 subtype AIV. Assays were performed at a single temperature (39 °C), and the results were obtained within 20 min.

Results

The assay showed no cross-detection with Newcastle disease virus or infectious bronchitis virus. The analytical sensitivity was 103 RNA copies/μL at a 95% confidence interval according to probit regression analysis, with 100% specificity. Compared with published reverse transcription quantitative real-time polymerase chain reaction assays, the κ value of the RT-RAA assay in 420 avian clinical samples was 0.983 (p < 0.001). The sensitivity for avian clinical sample detection was 97.26% (95% CI, 89.56–99.52%), and the specificity was 100% (95% CI, 98.64–100%).

Conclusions

These results indicated that our RT-RAA assay may be a valuable tool for detecting H5 subtype AIV.
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Metadata
Title
Reverse transcription recombinase-aided amplification assay for H5 subtype avian influenza virus
Authors
Suchun Wang
Yang Li
Fuyou Zhang
Nan Jiang
Qingye Zhuang
Guangyu Hou
Lijian Jiang
Jianmin Yu
Xiaohui Yu
Hualei Liu
Chenglong Zhao
Liping Yuan
Baoxu Huang
Kaicheng Wang
Publication date
01-12-2022
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2022
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/s12985-022-01807-0

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