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Open Access 01-12-2020 | Polymerase Chain Reaction | Research

Viral metagenomics revealed diverse CRESS-DNA virus genomes in faeces of forest musk deer

Authors: Qi Liu, Hao Wang, Yu Ling, Shi-Xing Yang, Xiao-Chun Wang, Rui Zhou, Yu-Qing Xiao, Xu Chen, Jie Yang, Wei-Guo Fu, Wen Zhang, Gui-Lan Qi

Published in: Virology Journal | Issue 1/2020

Abstract

Background

Musk deer can produce musk which has high medicinal value and is closely related to human health. Viruses in forest musk deer both threaten the health of forest musk deer and human beings.

Methods

Using viral metagenomics we investigated the virome in 85 faeces samples collected from forest musk deer.

Results

In this article, eight novel CRESS-DNA viruses were characterized, whole genomes were 2148 nt–3852 nt in length. Phylogenetic analysis indicated that some viral genomes were part of four different groups of CRESS-DNA virus belonging in the unclassified CRESS-DNA virus, Smacoviridae, pCPa-like virus and pPAPh2-like virus. UJSL001 (MN621482), UJSL003 (MN621469) and UJSL017 (MN621476) fall into the branch of unclassified CRESS-DNA virus (CRESSV1–2), UJSL002 (MN621468), UJSL004 (MN621481) and UJSL007 (MN621470) belong to the cluster of Smacoviridae, UJSL005 (MN604398) showing close relationship with pCPa-like (pCRESS4–8) clusters and UJSL006 (MN621480) clustered into the branch of pPAPh2-like (pCRESS9) virus, respectively.

Conclusion

The virome in faeces samples of forest musk deer from Chengdu, Sichuan province, China was revealed, which further characterized the diversity of viruses in forest musk deer intestinal tract.

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Title: Viral metagenomics revealed diverse CRESS-DNA virus genomes in faeces of forest musk deer
Authors: Qi Liu
Hao Wang
Yu Ling
Shi-Xing Yang
Xiao-Chun Wang
Rui Zhou
Yu-Qing Xiao
Xu Chen
Jie Yang
Wei-Guo Fu
Wen Zhang
Gui-Lan Qi
Publication date: 01-12-2020
Publisher: BioMed Central
Keyword: Polymerase Chain Reaction
Published in: Virology Journal / Issue 1/2020
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-020-01332-y

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Viral metagenomics revealed diverse CRESS-DNA virus genomes in faeces of forest musk deer

Abstract

Background

Methods

Results

Conclusion

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Abstract

Background

Methods

Results

Conclusion

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