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Journal of Translational Medicine

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Open Access 01-12-2019 | Colorectal Cancer | Research

Integration analysis for novel lncRNA markers predicting tumor recurrence in human colon adenocarcinoma

Authors: Fangyao Chen, Zhe Li, Changyu Deng, Hong Yan

Published in: Journal of Translational Medicine | Issue 1/2019

Abstract

Background

Numerous evidence has suggested that long non-coding RNA (lncRNA) acts an important role in tumor biology. This study focuses on the identification of novel prognostic lncRNA biomarkers predicting tumor recurrence in human colon adenocarcinoma.

Methods

We obtained the research data from The Cancer Genome Atlas (TCGA) database. The interaction among different expressed lncRNA, miRNA and mRNA markers between colon adenocarcinoma patients with and without tumor recurrence were verified with miRcode, starBase and miRTarBase databases. We established the lncRNA–miRNA–mRNA competing endogenous RNA (ceRNA) network based on the verified association between the selected markers. We performed the functional enrichment analysis to obtain better understanding of the selected lncRNAs. Then we use multivariate logistic regression to identify the prognostic lncRNA markers with covariates. We also generated a nomogram predicting tumor recurrence risk based on the identified lncRNA biomarkers and clinical covariates.

Results

We included 12,727 lncRNA, 1881 miRNA and 47,761 mRNA profiling and clinical features for 113 colon adenocarcinoma patients obtained from the TCGA database. After filtration, we used 37 specific lncRNAs, 60 miRNAs and 148 mRNAs in the ceRNA network analysis. We identified five lncRNAs as prognostic lncRNA markers predicting tumor recurrence in colon adenocarcinoma, in which four of them were identified for the first time. Finally, we generated a nomogram illustrating the association between the identified lncRNAs and the tumor recurrence risk in colon adenocarcinoma.

Conclusions

The four newly identified lncRNA biomarkers might be potential prognostic biomarkers predicting tumor recurrence in colon adenocarcinoma. We recommend that further clinical and fundamental researches be conducted on the identified lncRNA markers.

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Title: Integration analysis for novel lncRNA markers predicting tumor recurrence in human colon adenocarcinoma
Authors: Fangyao Chen
Zhe Li
Changyu Deng
Hong Yan
Publication date: 01-12-2019
Publisher: BioMed Central
Keywords: Colorectal Cancer
Colorectal Cancer
Published in: Journal of Translational Medicine / Issue 1/2019
Electronic ISSN: 1479-5876
DOI: https://doi.org/10.1186/s12967-019-2049-2

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Integration analysis for novel lncRNA markers predicting tumor recurrence in human colon adenocarcinoma

Abstract

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Methods

Results

Conclusions

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Abstract

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