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Journal of Translational Medicine
Published in: Journal of Translational Medicine 1/2019

Open Access 01-12-2019 | Diabetic Nephropathy | Research

The circulating exosomal microRNAs related to albuminuria in patients with diabetic nephropathy

Authors: Hyoungnae Kim, Yun-Ui Bae, Jin Seok Jeon, Hyunjin Noh, Hyeong Kyu Park, Dong Won Byun, Dong Cheol Han, Seongho Ryu, Soon Hyo Kwon

Published in: Journal of Translational Medicine | Issue 1/2019

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Abstract

Background

Diabetic nephropathy (DN) is associated with high risk of cardiovascular disease and mortality. Exosomal microRNAs (miRNAs) regulate gene expression in a variety of tissues and play important roles in the pathology of various diseases. We hypothesized that the exosomal miRNA profile would differ between DN patients and patients without nephropathy.

Methods

We prospectively enrolled 74 participants, including healthy volunteers (HVs), diabetic patients without nephropathy, and those with DN. The serum exosomal miRNA profiles of participants were examined using RNA sequencing.

Results

The expression levels of 107 miRNAs differed between HVs and patients without DN, whereas the expression levels of 95 miRNAs differed between HVs and patients with DN. Among these miRNAs, we found 7 miRNAs (miR-1246, miR-642a-3p, let-7c-5p, miR-1255b-5p, let-7i-3p, miR-5010-5p, miR-150-3p) that were uniquely up-regulated in DN patients compared to HVs, and miR-4449 that was highly expressed in DN patients compared to patients without DN. A pathway analysis revealed that these eight miRNAs are likely involved in MAPK signaling, integrin function in angiogenesis, and regulation of the AP-1 transcription factor. Moreover, they were all significantly correlated with the degree of albuminuria.

Conclusions

Patients with DN have a different serum exosomal miRNA profile compared to HVs. These miRNAs may be promising candidates for the diagnosis and treatment of DN and cardiovascular disease.
Appendix
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Metadata
Title
The circulating exosomal microRNAs related to albuminuria in patients with diabetic nephropathy
Authors
Hyoungnae Kim
Yun-Ui Bae
Jin Seok Jeon
Hyunjin Noh
Hyeong Kyu Park
Dong Won Byun
Dong Cheol Han
Seongho Ryu
Soon Hyo Kwon
Publication date
01-12-2019
Publisher
BioMed Central
Published in
Journal of Translational Medicine / Issue 1/2019
Electronic ISSN: 1479-5876
DOI
https://doi.org/10.1186/s12967-019-1983-3

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