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Cancer Cell International
Published in: Cancer Cell International 1/2018

Open Access 01-12-2018 | Primary Research

LncRNA XIST/miR-200c regulates the stemness properties and tumourigenicity of human bladder cancer stem cell-like cells

Authors: Ran Xu, Xuan Zhu, Fangzhi Chen, Changkun Huang, Kai Ai, Hongtao Wu, Lei Zhang, Xiaokun Zhao

Published in: Cancer Cell International | Issue 1/2018

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Abstract

Background

The abnormal expression of non-coding RNAs (ncRNAs), such as microRNAs and long ncRNAs, often contribute to the development of cancers. miR-200c functions as a tumour suppressor that impacts the growth of bladder cancer cells and the epithelial-to-mesenchymal transition (EMT). LncRNA X inactive specific transcript (XIST) is highly expressed in tumour tissues, promotes cancer progression and might act as an miRNA molecular sponge. This study aimed to examine the relationship between lncRNA XIST and miR-200c and to assess their functions in the regulation of the stemness properties and tumourigenicity of human bladder cancer stem cell (BCSC)-like cells.

Methods

Biological effects including cell clone formation, sphere formation, self-renewal properties and mouse tumourigenesis were examined in BCSC-like cells with miR-200c overexpression or XIST knockdown. Real-time PCR and western blotting were used to detect the expression changing of related factors in BCSC-like cells gene models. Dual luciferase reporter assay was used to examine the changes of XIST and miR-200c expression levels.

Results

The results indicated that miR-200c overexpression and XIST knockdown could inhibit cell clone formation, self-renewal ability and EMT in BCSC-like cells. miR-200c knockdown could restore the tumour growth inhibition caused by XIST knockdown.

Conclusion

LncRNA XIST may act as an inhibitor of miR-200c to regulate the stemness properties and tumourigenicity of bladder cancer cells, and our findings might reveal a potential strategy of targeting XIST for bladder cancer therapy.
Appendix
Available only for authorised users
Literature
1.
Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray F. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 2015;136(5):E359.CrossRefPubMed
2.
Tran MN, Goodwin JG, Mcconkey DJ, Kamat AM. Bladder cancer stem cells. Curr Stem Cell Res Ther. 2010;5(4):387–95.CrossRefPubMed
3.
Liu SL, Qi L, Han WQ, Zhu BS, Zhou X, Jiang SS, Chen MF, Li Y, He W, Liu LF. Shorter hemodialysis duration is a risk factor for the recurrence of urothelial carcinoma of the bladder in patients on maintenance hemodialysis. Clin Transl Oncol. 2016;18(3):304–9.CrossRefPubMed
4.
5.
Pishkari S, Paryan M, Hashemi M, Baldini E, Mohammadi-Yeganeh S. The role of microRNAs in different types of thyroid carcinoma: a comprehensive analysis to find new miRNA supplementary therapies. J Endocrinol Investig. 2017;2:1–15.
6.
7.
Tang Y, He R, An J, Deng P, Huang L, Yang W. lncRNA XIST interacts with miR-140 to modulate lung cancer growth by targeting iASPP. Oncol Rep. 2017;38(2):941.CrossRefPubMed
8.
Dermani FK, Saidijam M, Amini R, Mahdavinezhad A, Heydari K, Najafi R. Resveratrol inhibits proliferation, invasion, and epithelial–mesenchymal transition by increasing miR-200c expression in HCT-116 colorectal cancer cells. J Cell Biochem. 2017;118(6):1547–55.CrossRef
9.
Leskelä S, Leandro-García LJ, Mendiola M, Barriuso J, Inglada-Pérez L, Muñoz I, Martínez-Delgado B, Redondo A, De SJ, Robledo M. The miR-200 family controls beta-tubulin III expression and is associated with paclitaxel-based treatment response and progression-free survival in ovarian cancer patients. Endocr Relat Cancer. 2010;18(1):85.CrossRefPubMed
10.
Shimono Y, Zabala M, Cho RW, Lobo N, Dalerba P, Qian D, Diehn M, Liu H, Panula SP, Chiao E. Downregulation of miRNA-200c links breast cancer stem cells with normal stem cells. Cell. 2009;138(3):592–603.CrossRefPubMedPubMedCentral
11.
Vallejo DM, Caparros E, Dominguez M. Targeting Notch signalling by the conserved miR-8/200 microRNA family in development and cancer cells. EMBO J. 2011;30(4):756–69.CrossRefPubMedPubMedCentral
12.
Feng X, Hua H, Wen H, Lin Y, Luo S, Qian D, Duan R. Decreased expression of MicroRNA-200 family in human breast cancer is associated with lymph node metastasis. Clin Transl Oncol. 2016;18(3):283–8.CrossRef
13.
Bayoumi AS, Sayed A, Broskova Z, Teoh JP, Wilson J, Su H, Tang YL, Kim IM. Crosstalk between long noncoding RNAs and MicroRNAs in health and disease. Int J Mol Sci. 2016;17(3):356.CrossRefPubMedPubMedCentral
14.
Zhou D, Tong S, Hacisuleyman E, Teng F, Wang X, Brown M, Rinn JL, Lee GS, Chen Y, Kantoff PW. Integrative analyses reveal a long noncoding RNA-mediated sponge regulatory network in prostate cancer. Nat Commun. 2016;7:10982.CrossRef
15.
Engreitz JM, Pandyajones A, Mcdonel P, Shishkin A, Sirokman K, Surka C, Kadri S, Xing J, Goren A, Lander ES. The Xist lncRNA exploits three-dimensional genome architecture to spread across the X chromosome. Science. 2013;341(6147):1237973.CrossRefPubMedPubMedCentral
16.
Yao Y, Ma J, Xue Y, Wang P, Li Z, Liu J, Chen L, Xi Z, Teng H, Wang Z. Knockdown of long non-coding RNA XIST exerts tumor-suppressive functions in human glioblastoma stem cells by up-regulating miR-152. Cancer Lett. 2015;359(1):75.CrossRefPubMed
17.
Du P, Zhao H, Peng R, Liu Q, Yuan J, Peng G, Liao Y. LncRNA-XIST interacts with miR-29c to modulate the chemoresistance of glioma cell to TMZ through DNA mismatch repair (MMR) pathway. Biosci Rep. 2017;37(5):BSR20170696.CrossRefPubMedPubMedCentral
18.
Ren C, Li X, Wang T, Wang G, Zhao C, Liang T, Zhu Y, Li M, Yang C, Zhao Y. Functions and mechanisms of long noncoding RNAs in ovarian cancer. Int J Gynecol Cancer Off J Int Gynecol Cancer Soc. 2015;25(4):566.CrossRef
19.
Ponti D, Costa AN, Pratesi G, Petrangolini G, Coradini D, Pilotti S, Pierotti MA, Daidone MG. Isolation and in vitro propagation of tumorigenic breast cancer cells with stem/progenitor cell properties. Can Res. 2005;65(13):5506–11.CrossRef
20.
Yamamura S, Imaisumida M, Tanaka Y, Dahiya R. Interaction and cross-talk between non-coding RNAs. Cell Mol Life Sci. 2017;415–426:1–18.
21.
Chen DL, Ju HQ, Lu YX, Chen LZ, Zeng ZL, Zhang DS, Luo HY, Wang F, Qiu MZ, Wang DS. Long non-coding RNA XIST regulates gastric cancer progression by acting as a molecular sponge of miR-101 to modulate EZH2 expression. J Exp Clin Cancer Res. 2016;35(1):142.CrossRefPubMedPubMedCentral
22.
Kumar S, Nag A, Mandal CC. A comprehensive review on miR-200c, a promising cancer biomarker with therapeutic potential. Curr Drug Targets. 2015;16(12):1381–403.CrossRefPubMed
23.
Reya T, Morrison SJ, Clarke MF, Weissman IL. Stem cells, cancer, and cancer stem cells. Nature. 2001;414(6859):105.CrossRefPubMed
24.
Smr W, Islam F, Gopalan V, Lam AK. The identifications and clinical implications of cancer stem cells in colorectal cancer. Clin Colorectal Cancer. 2017;16(2):93.CrossRef
25.
Zhu YT, Zhao Z, Fu XY, Luo Y, Lei CY, Chen W, Li F, Pang SY, Chen SS, Tan WL. The granulocyte macrophage-colony stimulating factor surface modified MB49 bladder cancer stem cells vaccine against metastatic bladder cancer. Stem Cell Res. 2014;13(1):111–22.CrossRefPubMed
26.
Masferrer E, Ferrándizpulido C, Masferrerniubò M, Rodríguezrodríguez A, Gil I, Pont A, Servitje O, García DHA, Lloveras B, Garcíapatos V. Epithelial-to-mesenchymal transition in penile squamous cell carcinoma. J Urol. 2015;193(2):699–705.CrossRefPubMed
27.
28.
Liu L, Qiu M, Tan G, Liang Z, Qin Y, Chen L, Chen H, Liu J. miR-200c Inhibits invasion, migration and proliferation of bladder cancer cells through down-regulation of BMI-1 and E2F3. J Transl Med. 2014;12(1):305.CrossRefPubMedPubMedCentral
29.
Singh S, Sadacharan S, Su S, Belldegrun A, Persad S, Singh G. Overexpression of vimentin role in the invasive phenotype in an androgen-independent model of prostate cancer. Can Res. 2003;63(9):2306–11.
30.
Lei M, Zhou Y, Luo X, Hai G, Deng X, Jiang Y. Long non-coding RNA XIST promotes cell growth and invasion through regulating miR-497/MACC1 axis in gastric cancer. Oncotarget. 2017;8(3):4125.
31.
Thomson DW, Dinger ME. Endogenous microRNA sponges: evidence and controversy. Nat Rev Genet. 2016;17(5):272.CrossRefPubMed
32.
Fang J, Sun CC, Gong C. Long noncoding RNA XIST acts as an oncogene in non-small cell lung cancer by epigenetically repressing KLF2 expression. Biochem Biophys Res Commun. 2016;478(2):811–7.CrossRefPubMed
Metadata
Title
LncRNA XIST/miR-200c regulates the stemness properties and tumourigenicity of human bladder cancer stem cell-like cells
Authors
Ran Xu
Xuan Zhu
Fangzhi Chen
Changkun Huang
Kai Ai
Hongtao Wu
Lei Zhang
Xiaokun Zhao
Publication date
01-12-2018
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2018
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-018-0540-0

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