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Journal of Experimental & Clinical Cancer Research
Published in: Journal of Experimental & Clinical Cancer Research 1/2016

Open Access 01-12-2016 | Research

Long non-coding RNA XIST regulates gastric cancer progression by acting as a molecular sponge of miR-101 to modulate EZH2 expression

Authors: Dong-liang Chen, Huai-qiang Ju, Yun-xin Lu, Le-zong Chen, Zhao-lei Zeng, Dong-sheng Zhang, Hui-yan Luo, Feng Wang, Miao-zhen Qiu, De-shen Wang, Da-zhi Xu, Zhi-wei Zhou, Helene Pelicano, Peng Huang, Dan Xie, Feng-hua Wang, Yu-hong Li, Rui-hua Xu

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2016

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Abstract

Background

Long non-coding RNAs (lncRNAs) have emerged as critical regulators of tumor progression. However, the role and molecular mechanism of lncRNA XIST in gastric cancer is still unknown.

Methods

Real-time PCR analysis was performed to measure the expression levels of lncRNA XIST in gastric cancer tissues and cell lines, the correlation between lncRNA XIST expression and clinicopathological characteristics and prognosis was analyzed in gastric cancer patients. The biological function of lncRNA XIST on gastric cancer cells were determined both in vitro and in vivo. The regulating relationship between lncRNA XIST and miR-101 was investigated in gastric cancer cells.

Results

lncRNA XIST was significantly up-regulated in gastric cancer tissues and cell lines. Overexpression of lncRNA XIST was markedly associated with larger tumor size, lymph node invasion, distant metastasis and TNM stage in gastric cancer patients. Functionally, knockdown of lncRNA XIST exerted tumor-suppressive effects by inhibiting cell proliferation, migration and invasion in vitro and tumor growth and metastasis in vivo. Furthermore, an inverse relationship between lncRNA XIST and miR-101 was found. Polycomb group protein enhancer of zeste homolog 2 (EZH2), a direct target of miR-101, could mediated the biological effects that lncRNA XIST exerted.

Conclusions

lncRNA XIST is up-regulated and is associated with aggressive tumor phenotypes and patient survival in gastric cancer, and the newly identified lncRNA XIST/miR-101/EZH2 axis could be a potential biomarkers or therapeutic targets for gastric cancer patients.
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Metadata
Title
Long non-coding RNA XIST regulates gastric cancer progression by acting as a molecular sponge of miR-101 to modulate EZH2 expression
Authors
Dong-liang Chen
Huai-qiang Ju
Yun-xin Lu
Le-zong Chen
Zhao-lei Zeng
Dong-sheng Zhang
Hui-yan Luo
Feng Wang
Miao-zhen Qiu
De-shen Wang
Da-zhi Xu
Zhi-wei Zhou
Helene Pelicano
Peng Huang
Dan Xie
Feng-hua Wang
Yu-hong Li
Rui-hua Xu
Publication date
01-12-2016
Publisher
BioMed Central
Published in
Journal of Experimental & Clinical Cancer Research / Issue 1/2016
Electronic ISSN: 1756-9966
DOI
https://doi.org/10.1186/s13046-016-0420-1

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