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BMC Musculoskeletal Disorders
Published in: BMC Musculoskeletal Disorders 1/2015

Open Access 01-12-2015 | Research article

A new animal model for delayed osseous union secondary to osteitis

Authors: Lars Helbig, Thorsten Guehring, Svenja Rosenberger, Adriana Ivanova, Kathrin Kaeppler, Christian Alexander Fischer, Arash Moghaddam, Gerhard Schmidmaier

Published in: BMC Musculoskeletal Disorders | Issue 1/2015

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Abstract

Background

The treatment of infection-related delayed bone unions is still very challenging for the orthopedic surgeon. The prevalence of such infection-related types of osteitis is high in complex fractures, particularly in open fractures with extensive soft-tissue damage. The aim of this study was to develop a new animal model for delayed union due to osteitis.

Methods

After randomization to infected or non-infected groups 20 Sprague–Dawley rats underwent a transverse fracture of the midshaft tibia. After intramedullary inoculation with staphylococcus aureus (103 CFU) fracture stabilization was done by intramedullary titanium K-wires. After 5 weeks all rats were euthanized and underwent biomechanical testing to evaluate bone consolidation or delayed union, respectively. Micro-CT scans were additionally used to quantitatively evaluate the callus formation by the score of Lane and Sandhu. Blood samples were taken to analyze infectious disease markers (day 1, 14 and 35).

Results

Biomechanical testing showed a significant higher maximum torque in the non-infected group 5 weeks postoperatively compared with the infected group (p < 0.001). According to the Lane and Sandhu score a significantly higher callus formation was found in the non-infected group (p < 0.001). Similarly, the leucocyte count in the infected group was significantly higher than in the non-infected group (p < 0.05).

Conclusions

Here we have established a new animal model for delayed osseous union secondary to osteitis. The animal model appears to be appropriate for future experimental studies to test new therapeutic strategies in these difficult to treat bone healing complications.
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Metadata
Title
A new animal model for delayed osseous union secondary to osteitis
Authors
Lars Helbig
Thorsten Guehring
Svenja Rosenberger
Adriana Ivanova
Kathrin Kaeppler
Christian Alexander Fischer
Arash Moghaddam
Gerhard Schmidmaier
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Musculoskeletal Disorders / Issue 1/2015
Electronic ISSN: 1471-2474
DOI
https://doi.org/10.1186/s12891-015-0816-7

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