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BMC Cardiovascular Disorders

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Open Access 01-12-2020 | Implantable Cardioverter Defibrillator | Research article

Novel myocardial markers GADD45G and NDUFS5 identified by RNA-sequencing predicts left ventricular reverse remodeling in advanced non-ischemic heart failure: a retrospective cohort study

Authors: Togo Iwahana, Sho Okada, Masato Kanda, Motohiko Oshima, Atsushi Iwama, Goro Matsumiya, Yoshio Kobayashi

Published in: BMC Cardiovascular Disorders | Issue 1/2020

Abstract

Background

Left ventricular reverse remodeling (LVRR) has been detected in non-ischemic dilated cardiomyopathy (NIDCM) patients following optimal treatment. However, its prediction with only conventional modalities is often difficult. This study sought to examine whether RNA sequencing (RNA-seq) of myocardium tissue samples could predict LVRR in NIDCM.

Methods

A total of 17 advanced NIDCM patients with left ventricular ejection fraction (LVEF) below 30% who underwent cardiac biopsy from Left ventricle (LV) were prospectively recruited. They received optimal treatment and followed with echocardiogram every 6 months. Based on LVRR status after 12 months of treatment, patients were divided into the reverse remodeling (RR) or non-RR group. Tissue samples were analyzed by RNA-seq, and a functional analysis of differentially expressed genes was carried out.

Results

There were eight and nine patients in the RR and non-RR groups, respectively. No difference was found in age, sex, disease duration, LV end-diastolic diameter, and LVEF between the two groups. There were 155 genes that were differentially expressed between the two groups. Nicotinamide adenine dinucleotide ubiquinone oxidoreductase subunit (NDUF)S5 and Growth arrest and DNA-damage-inducible protein (GADD)45G, along with several genes related to the mitochondrial respiratory chain and ribosome, were significantly downregulated in the RR as compared to the non-RR group.

Conclusion

GADD45G and NDUFS5 are potential biomarkers for LVRR in patients with advanced NIDCM.

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Title: Novel myocardial markers GADD45G and NDUFS5 identified by RNA-sequencing predicts left ventricular reverse remodeling in advanced non-ischemic heart failure: a retrospective cohort study
Authors: Togo Iwahana
Sho Okada
Masato Kanda
Motohiko Oshima
Atsushi Iwama
Goro Matsumiya
Yoshio Kobayashi
Publication date: 01-12-2020
Publisher: BioMed Central
Keywords: Implantable Cardioverter Defibrillator
Heart Failure
Published in: BMC Cardiovascular Disorders / Issue 1/2020
Electronic ISSN: 1471-2261
DOI: https://doi.org/10.1186/s12872-020-01396-2

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Novel myocardial markers GADD45G and NDUFS5 identified by RNA-sequencing predicts left ventricular reverse remodeling in advanced non-ischemic heart failure: a retrospective cohort study

Abstract

Background

Methods

Results

Conclusion

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Abstract

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Conclusion

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