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BMC Cardiovascular Disorders

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Open Access 01-12-2017 | Research article

Cardiac tissue oxidative stress and inflammation after vitamin D administrations in high fat- diet induced obese rats

Authors: Mahdieh Abbasalizad Farhangi, Ghazaleh Nameni, Ghazaleh Hajiluian, Mehran Mesgari-Abbasi

Published in: BMC Cardiovascular Disorders | Issue 1/2017

Abstract

Background

Obesity is associated with numerous metabolic and inflammatory disorders. The current study was aimed to evaluate the effects of vitamin D administration on the markers of oxidative stress and inflammation in the cardiac tissue of high-fat diet induced obese rats.

Methods

In the beginning of the study, 40 male Wistar rats were divided into two groups: normal diet (ND) and high fat diet (HFD) for 16 weeks; then each group subdivided into two groups including: ND, ND + vitamin D, HFD and HFD + vitamin D. Vitamin D supplementation was done for 5 weeks at 500 IU/kg dosage. Tumor necrosis factor (TNF)-α concentration and markers of oxidative stress including glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA) and catalase (CAT) concentrations in the cardiac tissue and serum concentrations of lipids in rats were determined using ELISA kits and spectrophotometry methods respectively.

Results

According to our results, GPx activity in ND and ND + vitamin D group was significantly higher compared with HFD group. Similarly, SOD activity was also significantly increased in ND + vitamin D group compared with ND and HFD groups. Moreover, vitamin D administration, significantly reduced catalase activity in ND + vitamin D and HFD + vitamin D groups (P < 0.05). TNF-α concentration in heart tissue in ND + vitamin D group significantly reduced compared with ND group. Cardiac tissue MDA concentration in baseline or after vitamin D administration did not changed significantly.

Conclusion

Vitamin D improved cardiac oxidative stress and inflammatory markers in HFD induced obese rats. Further studies in human models are needed to further confirm the use of this nutrient in daily clinical practice.

Fernandez-Sanchez A, Madrigal-Santillan E, Bautista M, Esquivel-Soto J, Morales-González A, Esquivel-Chirino C, et al. Inflammation, oxidative stress, obesity. Int J Mol Sci. 2011;12(5):3117–32.CrossRefPubMedPubMedCentral

Miller AA, Spencer SJ. Obesity and neuroinflammation: a pathway to cognitive impairment. Brain Behav Imunol. 2014;42:10–21.CrossRef

Ng M, Fleming T, Robinson M, Thomson B, Graetz N, Margono C. Global, regional, and national prevalence of overweight and obesity in children and adults during 1980–2013: a systematic analysis for the global burden of disease study 2013. Lancet. 2014;384(9945):766–81.CrossRefPubMedPubMedCentral

Komaki A, Karimi SA, Salehi I, Sarihi A, Shahidi S, Zarei M. The treatment combination of vitamins E and C and astaxanthin prevents high-fat diet induced memory deficits in rats. Pharacol Biochem. 2015;131(88):98–103.CrossRef

Gortmaker SL, Swinburn BA, Levy D, Carter R, Mabry PL, Finegood DT. Changing the future of obesity: science, policy, and action. Lancet. 2011;378(9793):838–47.CrossRefPubMedPubMedCentral

Hariri N, Thibault L. High-fat diet-induced obesity in animal models. NRR. 2010;23(2):270–99.CrossRef

Khaki Khatibi F, Yaghoubi A, Zarghami N, Rahbani M, Babaie H. Evaluation of hs-CRP, antioxidant markers and MDA in patients of coronary artery disease (CAD) containing non-smokers and non-diabetics. J Cardiovasc Thorac Res. 2011;2(4):13–8.

Khan N, Naz L, Yasmeen G. Obesity: an independent risk factor systemic oxidative stress. Park J Pharm Sci. 2006;19:62–9.

Ansari JA, Bhandari U, Pillai KK, Haque SE. Effect of rosuvastatin on obesity-induced cardiac oxidative stress in Wistar rats-a preliminary study. Indian J Exp Biol. 2012;50(3):216–22.PubMed

10.

Olorunnisola OS, Bradley G, Afolayan AJ. Protective effect of T. violacea rhizome extract against hypercholesterolemia-induced oxidative stress in Wistar rats. Molecules. 2012;17(5):6033–45.CrossRefPubMed

11.

Pezeshkian M, Darbin A, Rashidi MR, Vatankhah A, Golmohammadi Z, Afrasiabi A, et al. The effect of atherogenic diet with or without enzyme inhibitors on the incidence and progress of atherosclerosis in rabbits. J Cardiovasc Thorac Res. 2011;3(1):7–10.

12.

Sokhanvar S, Mazaki RRS, Mousavinasab N, Golmohammadi Z. The association between serum lipoprotein (a) and other cardiac risk factors with the severity of coronary artery disease. J Cardiovasc Thorac Res. 2011;3(1):35–9.

13.

Sharifi MH, Eftekhari MH, Ostovan MA, Rezaianazadeh A. Effects of a therapeutic lifestyle change diet and supplementation with Q10 plus L-carnitine on quality of life in patients with myocardial infarction: a randomized clinical trial. J Cardiovasc Thorac Res. 2017;9(1):21–8.CrossRefPubMedPubMedCentral

14.

Neale EP, Batterham MJ, Tapsell LC. Consumption of a healthy dietary pattern results in significant reductions in C-reactive protein levels in adults: a meta-analysis. Nutr Res. 2016;36(5):391–401.CrossRefPubMed

15.

Farhangi MA, Najafi M, Jafarabadi MA, Jahangiry L. Mediterranean dietary quality index and dietary phytochemical index among patients candidate for coronary artery bypass grafting (CABG) surgery. BMC Cardiovasc Disord. 2017;17:114–22.CrossRefPubMedPubMedCentral

16.

Noyan T, Balaharog R, Kömürog U. The oxidant and antioxidant effects of 25-hydroxyvitamin D3 in liver, kidney and heart tissues of diabetic rats. Clin Exp Med. 2005;5:31–6.CrossRefPubMed

17.

Tardif JC, Bourassa MG. Use of antioxidants in patients with heart failure. Antioxidants and cardiovascular disease. USA: Springer Science & Business Media; 2012. p. 213.

18.

Rodrigo R, Libuy M, Feliú F, Hasson D. The protective effect of lipoic acid on selected cardiovascular diseases caused by age-related oxidative stress. Oxid Med Cell Long. 2015; 2015:Article ID 313021.

19.

Borel P, Caillaud D, Cano NJ. Vitamin D bioavailability: state of the art. Crit Rev Food Sci Nutr. 2015;55:1193–205.CrossRefPubMed

20.

Walentowicz-Sadłecka M. W.P. Sadłecki P, Grabiec M. The role of vitamin D in the carcinogenesis of breast and ovarian cancer. Ginekol Pol. 2013;84(4):305–8.PubMed

21.

Wiseman H. Vitamin D is a membrane antioxidant Ability to inhibit iron-dependent lipid peroxidation in liposomes compared to cholesterol, ergosterol and tamoxifen and relevance to anticancer action. FEBS Lett 1993;326:285–288.

22.

Ke CY, Yang FL, Wu WT, Chung CH, Lee RP, Yang WT, et al. Vitamin D3 reduces tissue damage and oxidative stress caused by exhaustive exercise. Int J Med Sci. 2016;13(2):147–53.CrossRefPubMedPubMedCentral

23.

Murr C, Pilz S, Grammer TB, Kleber ME, Meinitzer A, Boehm BO. Vitamin D deficiency parallels inflammation and immune activation, the Ludwigshafen risk and cardiovascular health (LURIC) study. Clin Chem Lab Med. 2012;50(12):2205–12.CrossRefPubMed

24.

Argacha JF, Egrise D, Pochet S, Fontaine D, Lefort A, Libert F, et al. Vitamin D deficiency-induced hypertension is associated with vascular oxidative stress and altered heart gene expression. J Cardiovasc Pharmacol. 2011;58(1):65–71.CrossRefPubMed

25.

Gode S, Aksu T, Demirel A, Sunbul M, Gul M, Bakır I, et al. Effect of vitamin D deficiency on the development of postoperative atrial fibrillation in coronary artery bypass patients. J Cardiovasc Thorac Res. 2016;8(4):140–6.CrossRefPubMedPubMedCentral

26.

Danesh J, Wheeler JG, Hirschfield GM, Eda S, Eiriksdottir G, Rumley A, et al. C-reactive protein and other circulating markers of inflammation in the prediction of coronary heart disease. N Engl J Med. 2004;350(14):1387–97.CrossRefPubMed

27.

Barbero U, D'Ascenzo F, Nijhoff F, Moretti C, Biondi-Zoccai G, Mennuni M, et al. Assessing risk in patients with stable coronary disease: when should we intensify care and follow-Up? results from a meta-analysis of observational studies of the COURAGE and FAME Era. Scientifica (Cairo). 2016; 2016:3769152.

28.

Hajiluian G, Nameni G, Shahabi P, Mesgari-Abbasi M, Sadigh-Eteghad S, Farhangi MA. Vitamin D administration, cognitive function, BBB permeability and neuroinflammatory factors in high-fat diet-induced obese rats. Int J Obes (Lond). 2017; 41: 639–644.

29.

Nameni G, Hajiluian G, Shahabi P, Farhangi MA, Mesgari-Abbasi M, Hemmati MR. The impact of vitamin D supplementation on neurodegeneration, TNF-α concentration in hypothalamus, and CSF-to-plasma ratio of insulin in high-fat-diet-induced obese rats. J Mol Neurosci. 2016;61(2):247–55.CrossRefPubMed

30.

Nameni G, Farhangi MA, Hajilouian G, Shahabi P, Mesgari-Abbasi M. Insulin deficiency: a possible link between obesity and cognitive function. Int J Dev Neurosci. 2017;59:15–20.CrossRefPubMed

31.

Sobesky JL, Barrientos RM, Henning S, Thompson BM, Weber MD, Watkins LR. High-fat diet consumption disrupts memory and primes elevations in hippocampal IL-1β, an effect that can be prevented with dietary reversal or IL-1 receptor antagonism. Brain Behav Immun. 2014;42:22–32.CrossRefPubMed

32.

Erion JR, Wosiski-Kuhn M, Dey A, Hao S, Davis CL, Pollock NK. Obesity elicits interleukin 1-mediated deficits in hippocampal synaptic plasticity. J Neurosci. 2014;34(7):2618–31.CrossRefPubMedPubMedCentral

33.

Boitard C, Cavaroc A, Sauvant J, Aubert A, Castanon N, Layé S. Impairment of hippocampal-dependent memory induced by juvenile high-fat diet intake is associated with enhanced hippocampal inflammation in rats. Brain Behav Immun. 2014;40:9–17.CrossRefPubMed

34.

Pozzi F, Frajese GV, Frajese G. Vitamin D (Calcifediol) supplementation modulates NGF and BDNF and improves memory function in postmenopausal women: a pilot study. Endocrinol. 2013:1–11.

35.

Latimer CS, Brewer LD, Searcy JL, Chen K-C, Popović J, Kraner SD. Vitamin D prevents cognitive decline and enhances hippocampal synaptic function in aging rats. Proc Natl Acad Sci. 2014;111(41):E4359–E66.CrossRefPubMedPubMedCentral

36.

Noeman SA, Hamooda HE, Baalash AA. Biochemical study of oxidative stress markers in the liver, kidney and heart of high fat diet induced obesity in rats. Diabetol Metab Syn. 2011;3(1):17–25.CrossRef

37.

Hünkar T, Aktan F, Ceylan A, Karasu C. Effects of cod liver oil on tissue antioxidant pathways in normal and streptozotocin- diabetic rats (the ADIC study group). Cell Biochem Funct. 2002;20:297–302.CrossRefPubMed

38.

Maritim AC, Sanders RA, Watkins JB. Diabetes, oxidative stress and antioxidants: a review. J Biochem Mol Toxicol. 2003;17:24–38.CrossRefPubMed

39.

Tarcin O, Yavuz DG, Ozben B, Telli A, Ogunc AV, Yuksel M. Effect of vitamin D deficiency and replacement on endothelial function in asymptomatic subjects. J Clin Endocrinol Metab. 2009;94:4023–30.CrossRefPubMed

40.

Hirata M, Serizawa K, Aizawa K, Yogo K, Tashiro Y, Takeda S. 22-Oxacalcitriol prevents progression of endothelial dysfunction through antioxidative effects in rats with type 2 diabetes and early-stage nephropathy. Nephrol Dial Transplant. 2013;28:1166–74.CrossRefPubMed

41.

Polidoro L, Properzi G, Marampon F, Gravina GL, Festuccia C, Di Cesare E. Vitamin D protects human endothelial cells from H(2)O(2) oxidant injury through the Mek/Erk- Sirt1 axis activation. J Cardiovasc Transl Res. 2013;6:221–31.CrossRefPubMed

42.

Uberti F, Lattuada D, Morsanuto V, Nava U, Bolis G, Vacca G. Vitamin D protects human endothelial cells from oxidative stress through the autophagic and survival pathways. J Clin Endocrinol Metab. 2014;99:1367–74.CrossRefPubMed

43.

Wortsman J, Matsuoka LY, Chen TC, Lu Z, Holick MF. Decreased bioavailability of vitamin D in obesity. Am J Clin Nutr. 2007;72:690–3.

44.

McGill A-T, Stewart JM, Lithander FE, Strik CM, Poppitt SD. Relationships of low serum vitamin D3 with anthropometry and markers of the metabolic syndrome and diabetes in overweight and obesity. Nutr J. 2008;7:4–9.CrossRefPubMedPubMedCentral

45.

Al-Rasheed NM, Al-Rasheed NM, Bassiouni YA, Hasan IH, Al-Amin MA, Al-Ajmi HN, et al. Vitamin D attenuates pro-inflammatory TNF-α cytokine expression by inhibiting NF-кB/p65 signaling in hypertrophied rat hearts. J Physiol Biochem. 2015;71(2):289–99.CrossRefPubMed

46.

Zhu Y, Mahon BD, Froicu M, Cantorn MT. Calcium and 1 alpha,25-dihydroxyvitamin D target the TNF-alpha pathway to suppress experimental inflammatory bowel disease. Eur J Immunol. 2005;35:217–24.CrossRefPubMed

47.

Moller S, Laigaard F, Olgaard K, Hemmingsen C. Effect of 1, 25-dihydroxy-vitamin D3 in experimental sepsis. Int J Med Sci. 2007;4(4):190–5.CrossRefPubMedPubMedCentral

48.

Farhangi MA, Keshavarz SA, Eshraghian M, Ostadrahimi A, Saboor-Yaraghi AA. White blood cell count in women: relation to inflammatory biomarkers, haematological profiles, visceral adiposity, and other cardiovascular risk factors. J Health Popul Nutr. 2013;31(1):58–64.CrossRefPubMedPubMedCentral

49.

Buettner R, Schölmerich J, Bollheimer LC. High-fat diets: modeling the metabolic disorders of human obesity in rodents. Obesity. 2007;15(4):798–808.CrossRefPubMed

50.

McGill AT, Stewart JM, Lithander FE, Strik CM, Poppitt SD. Relationships of low serum vitamin D 3 with anthropometry and markers of the metabolic syndrome and diabetes in overweight and obesity. Nutr J. 2008;7(1):4.CrossRefPubMedPubMedCentral

51.

Asemi Z, Hashemi T, Karamali M, Samimi M, Esmaillzadeh A. Effects of vitamin D supplementation on glucose metabolism, lipid concentrations, inflammation, and oxidative stress in gestational diabetes: a double-blind randomized controlled clinical trial. Am J Clinical Nutr. 2013;98(6):1425–32.CrossRef

52.

Nagpal J, Pande JN, Bhartia A. A double-blind, randomized, placebo-controlled trial of the short-term effect of vitamin D3 supplementation on insulin sensitivity in apparently healthy, middle-aged, centrally obese men. Diab Med. 2009;26(1):19–27.CrossRef

Title: Cardiac tissue oxidative stress and inflammation after vitamin D administrations in high fat- diet induced obese rats
Authors: Mahdieh Abbasalizad Farhangi
Ghazaleh Nameni
Ghazaleh Hajiluian
Mehran Mesgari-Abbasi
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: BMC Cardiovascular Disorders / Issue 1/2017
Electronic ISSN: 1471-2261
DOI: https://doi.org/10.1186/s12872-017-0597-z

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Conclusion

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