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Open Access 01-12-2015 | Research article

Exercise training restores the cardiac microRNA-1 and −214 levels regulating Ca²⁺ handling after myocardial infarction

Authors: Stéphano Freitas Soares Melo, Valério Garrone Barauna, Vander José Neves, Tiago Fernandes, Lucienne da Silva Lara, Diego Robles Mazzotti, Edilamar Menezes Oliveira

Published in: BMC Cardiovascular Disorders | Issue 1/2015

Abstract

Background

Impaired cardiomyocyte contractility and calcium handling are hallmarks of left ventricular contractile dysfunction. Exercise training has been used as a remarkable strategy in the treatment of heart disease. The microRNA-1, which targets sodium/calcium exchanger 1 (NCX), and microRNA-214, which targets sarcoplasmic reticulum calcium ATPase-2a (Serca2a), are involved in cardiac function regulation. Thus, the aim of this study was to evaluate the effect of exercise training on cardiac microRNA-1 and −214 expression after myocardial infarction.

Methods

Wistar rats were randomized into four groups: sedentary sham (S-SHAM), sedentary infarction (S-INF), trained sham (T-SHAM), and trained infarction (T-INF). Exercise training consisted of 60 min/days, 5 days/week for 10 weeks with 3 % of body weight as overload beginning four weeks after myocardial infarction.

Results

MicroRNA-1 and −214 expressions were, respectively, decreased (52 %) and increased (54 %) in the S-INF compared to the S-SHAM, while exercise training normalized the expression of these microRNAs. The microRNA targets NCX and Serca-2a protein expression were, respectively, decreased (55 %) and increased (34 %) in the T-INF group compared to the S-INF group.

Conclusions

These results suggest that exercise training restores microRNA-1 and −214 expression levels and prevents change in both NCX and Serca-2a protein and gene expressions. Altogether, our data suggest a molecular mechanism to restore ventricular function after exercise training in myocardial infarction rats.

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Title: Exercise training restores the cardiac microRNA-1 and −214 levels regulating Ca2+ handling after myocardial infarction
Authors: Stéphano Freitas Soares Melo
Valério Garrone Barauna
Vander José Neves
Tiago Fernandes
Lucienne da Silva Lara
Diego Robles Mazzotti
Edilamar Menezes Oliveira
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: BMC Cardiovascular Disorders / Issue 1/2015
Electronic ISSN: 1471-2261
DOI: https://doi.org/10.1186/s12872-015-0156-4

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