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Open Access 01-12-2019 | Tuberculosis | Research article

IgA and IgG antibody detection of mycobacterial antigens in pleural fluid and serum from pleural tuberculous patients

Authors: Renan Jeremias da Silva, Raquel da Silva Corrêa, Isabela Gama Sardella, Ana Carla de Paulo Mulinari, Thiago Thomaz Mafort, Ana Paula Santos, Rogério Rufino, Luciana Silva Rodrigues, Maria Helena Féres Saad

Published in: BMC Immunology | Issue 1/2019

Abstract

Background

A previous study demonstrated pleural fluid (PF) IgA immunodominance for the fused MT10.3:MPT64 protein in pleural tuberculosis (PLTB) cases. However, no clue on the role of IgA and IgG against this and other antigens in PF and serum concerning improved diagnosis is available. Thus, the aim of the present study was to validate PF IgA-MT10.3:MPT64 and evaluate PF and serum IgA and IgG reactivity against this protein, its peptides (F2) and single MPT64, MT10.3 and the PPE59 mycobacterial specific antigens. IgA and IgG ELISA were measured against the antigen in PLTB (n = 29) and other non-TB pleurisy (n = 39) patient samples.

Results

The immunodominance of PF IgA-MT10.3:MPT64 was confirmed in PLTB (86.2%) followed by PPE59 (62%), while serum IgA-F2 exhibited 51.7% sensitivity. PF and serum IgG-MT10.3:MPT64 led to 65.5 and 51.7% sensitivity, respectively. However, MT10.3 and MPT64 displayed overall lower sensitivity (≤34.5) for both antibodies. All results at 95% fixed specificity. Combinatory results indicated 93.1% sensitivity for PF IgA-MT10.3:MPT64/−PPE59 and IgA/IgG-MT10.3:MPT64 at 92.3% specificity, followed by IgA-MT10.3:MPT64/−MPT64 or /−F2 (89.6%) without jeopardizing specificity (94.9%). The combinatory results of the PF adenosine deaminase test (ADA) and IgA-MT10.3:MPT64/−F2 demonstrated the highest sensitivity (96.6%), with a specificity of 92.3%.

Conclusions

The PF IgA-MT10:MPT64 immune dominance was validated in PLTB, and its combinatory results with PPE59 or MPT64 or F2 antigens as well as with IgG, are reported herein for the first time, improving their potential to assist diagnosis. Combining PF-ADA and IgA-MT10.3:MPT64/−F2 results achieved better accuracy. Moreover, serum IgG, although less accurate, displays potential beyond microbiological tests.

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Title: IgA and IgG antibody detection of mycobacterial antigens in pleural fluid and serum from pleural tuberculous patients
Authors: Renan Jeremias da Silva
Raquel da Silva Corrêa
Isabela Gama Sardella
Ana Carla de Paulo Mulinari
Thiago Thomaz Mafort
Ana Paula Santos
Rogério Rufino
Luciana Silva Rodrigues
Maria Helena Féres Saad
Publication date: 01-12-2019
Publisher: BioMed Central
Keywords: Tuberculosis
Mycobacterium Tuberculosis
Tuberculosis
Published in: BMC Immunology / Issue 1/2019
Electronic ISSN: 1471-2172
DOI: https://doi.org/10.1186/s12865-019-0315-y

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