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Journal of Experimental & Clinical Cancer Research

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Open Access 01-12-2011 | Research

The expression and role of protein kinase C (PKC) epsilon in clear cell renal cell carcinoma

Authors: Bin Huang, Kaiyuan Cao, Xiubo Li, Shengjie Guo, Xiaopeng Mao, Zhu Wang, Jintao Zhuang, Jincheng Pan, Chengqiang Mo, Junxing Chen, Shaopeng Qiu

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2011

Abstract

Protein kinase C epsilon (PKCε), an oncogene overexpressed in several human cancers, is involved in cell proliferation, migration, invasion, and survival. However, its roles in clear cell renal cell carcinoma (RCC) are unclear. This study aimed to investigate the functions of PKCε in RCC, especially in clear cell RCC, to determine the possibility of using it as a therapeutic target. By immunohistochemistry, we found that the expression of PKCε was up-regulated in RCCs and was associated with tumor Fuhrman grade and T stage in clear cell RCCs. Clone formation, wound healing, and Borden assays showed that down-regulating PKCε by RNA interference resulted in inhibition of the growth, migration, and invasion of clear cell RCC cell line 769P and, more importantly, sensitized cells to chemotherapeutic drugs as indicated by enhanced activity of caspase-3 in PKCε siRNA-transfected cells. These results indicate that the overexpression of PKCε is associated with an aggressive phenotype of clear cell RCC and may be a potential therapeutic target for this disease.

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Title: The expression and role of protein kinase C (PKC) epsilon in clear cell renal cell carcinoma
Authors: Bin Huang
Kaiyuan Cao
Xiubo Li
Shengjie Guo
Xiaopeng Mao
Zhu Wang
Jintao Zhuang
Jincheng Pan
Chengqiang Mo
Junxing Chen
Shaopeng Qiu
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: Journal of Experimental & Clinical Cancer Research / Issue 1/2011
Electronic ISSN: 1756-9966
DOI: https://doi.org/10.1186/1756-9966-30-88

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