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Journal of Experimental & Clinical Cancer Research

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Open Access 01-12-2011 | Research

Reduced expression of SMAD4 in gliomas correlates with progression and survival of patients

Authors: Shi-ming He, Zhen-wei Zhao, Yuan Wang, Ji-pei Zhao, Liang Wang, Fang Hou, Guo-dong Gao

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2011

Abstract

Background

To examine the expression of SMAD4 at gene and protein levels in glioma samples with different WHO grades and its association with survival.

Methods

Two hundreds fifty-two glioma specimens and 42 normal control tissues were collected. Immunochemistry assay, quantitative real-time PCR and Western blot analysis were carried out to investigate the expression of SMAD4. Kaplan-Meier method and Cox's proportional hazards model were used in survival analysis.

Results

Immunohistochemistry showed that SMAD4 expression was decreased in glioma. SMAD4 mRNA and protein levels were both lower in glioma compared to control on real-time PCR and Western blot analysis (both P < 0.001). In addition, its expression levels decrease from grade I to grade IV glioma according to the results of real-time PCR, immunohistochemistry analysis and Western blot. Moreover, the survival rate of SMAD4-positive patients was higher than that of SMAD4-negative patients. We further confirmed that the loss of SMAD4 was a significant and independent prognostic indicator in glioma by multivariate analysis.

Conclusions

Our data provides convincing evidence for the first time that the reduced expression of SMAD4 at gene and protein levels is correlated with poor outcome in patients with glioma. SMAD4 may play an inhibitive role during the development of glioma and may be a potential prognosis predictor of glioma.

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Title: Reduced expression of SMAD4 in gliomas correlates with progression and survival of patients
Authors: Shi-ming He
Zhen-wei Zhao
Yuan Wang
Ji-pei Zhao
Liang Wang
Fang Hou
Guo-dong Gao
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: Journal of Experimental & Clinical Cancer Research / Issue 1/2011
Electronic ISSN: 1756-9966
DOI: https://doi.org/10.1186/1756-9966-30-70

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