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Journal of Hematology & Oncology

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Open Access 01-12-2014 | Rapid communication

Differentially expressed microRNAs in the serum of cervical squamous cell carcinoma patients before and after surgery

Authors: Wen-Tao Wang, Ya-Nan Zhao, Jin-Xing Yan, Mei-Ying Weng, Yan Wang, Yue-Qin Chen, Shun-Jia Hong

Published in: Journal of Hematology & Oncology | Issue 1/2014

Abstract

Background

The purpose of this study was to detect the serum microRNAs (miRNAs) that are differentially expressed in cervical squamous cell carcinoma (SCC) patients and negative controls, with a focus on the miRNA profiles of the patients before and after surgery. The aim of the study is to evaluate the potential of these miRNAs as novel markers for the post-therapeutic monitoring of cervical SCC patients.

Results

A total of 765 serum miRNAs from 10 cervical SCC patients before surgery, 10 cervical SCC patients after surgery, and 10 negative controls were profiled using a TaqMan MicroRNA Array. A set of selected differentially expressed miRNAs were further analyzed in the patients at different perioperative periods, including preoperative, 1 week postoperative, and one month postoperative. The results showed that several serum miRNAs were differentially expressed in the cervical SCC patients compared with the negative controls, including miR-646, miR-141* and miR-542-3p. More importantly, we found that levels of specific serum miRNAs were deregulated in the pre- and postoperative stages, and these miRNAs could be useful for post-therapeutic monitoring of disease progression. Finally, we depicted a regulatory network of differentially expressed serum miRNAs, and many possible target genes were predicted in the estrogen-mediated signal pathways, supporting the hypothesis that cervical SCC is a hormone-associated gynecological disease.

Conclusions

Our study demonstrated that the circulating miRNAs miR-646, miR-141* and miR-542-3p could potentially serve as non-invasive biomarkers for cervical SCC. The levels of these specific miRNAs might be useful for the post-therapeutic monitoring of disease progression. This is the first report showing that circulating miRNAs could serve as biomarkers for the therapeutic intervention of cervical SCC.

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Walboomers JM, Jacobs MV, Manos MM, Bosch FX, Kummer JA, Shah KV, Snijders PJ, Peto J, Meijer CJ, Munoz N: Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. J Pathol. 1999, 189 (1): 12-19. 10.1002/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F.CrossRefPubMed

Castellsague X, Diaz M, de Sanjose S, Munoz N, Herrero R, Franceschi S, Peeling RW, Ashley R, Smith JS, Snijders PJ: Worldwide human papillomavirus etiology of cervical adenocarcinoma and its cofactors: implications for screening and prevention. J Natl Cancer Inst. 2006, 98 (5): 303-315. 10.1093/jnci/djj067.CrossRefPubMed

Forouzanfar MH, Foreman KJ, Delossantos AM, Lozano R, Lopez AD, Murray CJ, Naghavi M: Breast and cervical cancer in 187 countries between 1980 and 2010: a systematic analysis. Lancet. 2011, 378 (9801): 1461-1484. 10.1016/S0140-6736(11)61351-2.CrossRefPubMed

Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 2010, 127 (12): 2893-2917. 10.1002/ijc.25516.CrossRefPubMed

Bartel DP: MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004, 116: 281-297. 10.1016/S0092-8674(04)00045-5.CrossRefPubMed

Zhang H, Chen Z, Wang X, Huang Z, He Z, Chen Y: Long non-coding RNA: a new player in cancer. J Hematol Oncol. 2013, 6: 37-10.1186/1756-8722-6-37.PubMedCentralCrossRefPubMed

Hayashita Y, Osada H, Tatematsu Y, Yamada H, Yanagisawa K, Tomida S, Yatabe Y, Kawahara K, Sekido Y, Takahashi T: A polycistronic microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation. Cancer Res. 2005, 65 (21): 9628-9632. 10.1158/0008-5472.CAN-05-2352.CrossRefPubMed

Hassan O, Ahmad A, Sethi S, Sarkar FH: Recent updates on the role of microRNAs in prostate cancer. J Hematol Oncol. 2012, 5: 9-10.1186/1756-8722-5-9.PubMedCentralCrossRefPubMed

Akao Y, Nakagawa Y, Hirata I, Iio A, Itoh T, Kojima K, Nakashima R, Kitade Y, Naoe T: Role of anti-oncomirs miR-143 and -145 in human colorectal tumors. Cancer Gene Ther. 2010, 17 (6): 398-408. 10.1038/cgt.2009.88.CrossRefPubMed

10.

Sun YM, Lin KY, Chen YQ: Diverse functions of miR-125 family in different cell contexts. J Hematol Oncol. 2013, 6: 6-10.1186/1756-8722-6-6.PubMedCentralCrossRefPubMed

11.

Martinez I, Gardiner AS, Board KF, Monzon FA, Edwards RP, Khan SA: Human papillomavirus type 16 reduces the expression of microRNA-218 in cervical carcinoma cells. Oncogene. 2008, 27 (18): 2575-2582. 10.1038/sj.onc.1210919.PubMedCentralCrossRefPubMed

12.

Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, Peterson A, Noteboom J, O'Briant KC, Allen A: Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci U S A. 2008, 105 (30): 10513-10518. 10.1073/pnas.0804549105.PubMedCentralCrossRefPubMed

13.

Patel RS, Jakymiw A, Yao B, Pauley BA, Carcamo WC, Katz J, Cheng JQ, Chan EK: High resolution of microRNA signatures in human whole saliva. Arch Oral Biol. 2011, 56 (12): 1506-1513. 10.1016/j.archoralbio.2011.05.015.PubMedCentralCrossRefPubMed

14.

Wang G, Chan ES, Kwan BC, Li PK, Yip SK, Szeto CC, Ng CF: Expression of microRNAs in the urine of patients with bladder cancer. Clin Genitourin Cancer. 2012, 10 (2): 106-113. 10.1016/j.clgc.2012.01.001.CrossRefPubMed

15.

Roth C, Rack B, Muller V, Janni W, Pantel K, Schwarzenbach H: Circulating microRNAs as blood-based markers for patients with primary and metastatic breast cancer. Breast Cancer Res. 2010, 12 (6): R90-10.1186/bcr2766.PubMedCentralCrossRefPubMed

16.

Huang Z, Huang D, Ni S, Peng Z, Sheng W, Du X: Plasma microRNAs are promising novel biomarkers for early detection of colorectal cancer. Int J Cancer. 2010, 127 (1): 118-126. 10.1002/ijc.25007.CrossRefPubMed

17.

Heneghan HM, Miller N, Lowery AJ, Sweeney KJ, Newell J, Kerin MJ: Circulating microRNAs as novel minimally invasive biomarkers for breast cancer. Ann Surg. 2010, 251 (3): 499-505. 10.1097/SLA.0b013e3181cc939f.CrossRefPubMed

18.

Zhai H, Karaayvaz M, Dong P, Sakuragi N, Ju J: Prognostic significance of miR-194 in endometrial cancer. Biomark Res. 2013, 1: 12-10.1186/2050-7771-1-12.PubMedCentralCrossRefPubMed

19.

Hu Z, Chen X, Zhao Y, Tian T, Jin G, Shu Y, Chen Y, Xu L, Zen K, Zhang C, Shen H: Serum microRNA signatures identified in a genome-wide serum microRNA expression profiling predict survival of non-small-cell lung cancer. J Clin Oncol. 2010, 28 (10): 1721-1726. 10.1200/JCO.2009.24.9342.CrossRefPubMed

20.

Wang WT, Zhao YN, Han BW, Hong SJ, Chen YQ: Circulating microRNAs identified in a genome-wide serum microRNA expression analysis as noninvasive biomarkers for endometriosis. J Clin Endocrinol Metab. 2013, 98 (1): 281-289. 10.1210/jc.2012-2415.CrossRefPubMed

21.

Yu J, Wang Y, Dong R, Huang X, Ding S, Qiu H: Circulating microRNA-218 was reduced in cervical cancer and correlated with tumor invasion. J Cancer Res Clin Oncol. 2012, 138 (4): 671-674. 10.1007/s00432-012-1147-9.CrossRefPubMed

22.

Chen J, Yao D, Li Y, Chen H, He C, Ding N, Lu Y, Ou T, Zhao S, Li L, Long F: Serum microRNA expression levels can predict lymph node metastasis in patients with early-stage cervical squamous cell carcinoma. Int J Mol Med. 2013, 32 (3): 557-567.PubMedCentralPubMed

23.

Zhang Y, Jia Y, Zheng R, Guo Y, Wang Y, Guo H, Fei M, Sun S: Plasma microRNA-122 as a biomarker for viral-, alcohol-, and chemical-related hepatic diseases. Clin Chem. 2010, 56 (12): 1830-1838. 10.1373/clinchem.2010.147850.CrossRefPubMed

24.

Kuwabara Y, Ono K, Horie T, Nishi H, Nagao K, Kinoshita M, Watanabe S, Baba O, Kojima Y, Shizuta S: Increased microRNA-1 and microRNA-133a levels in serum of patients with cardiovascular disease indicate myocardial damage. Circ Cardiovasc Genet. 2011, 4 (4): 446-454. 10.1161/CIRCGENETICS.110.958975.CrossRefPubMed

25.

Deftereos G, Corrie SR, Feng Q, Morihara J, Stern J, Hawes SE, Kiviat NB: Expression of mir-21 and mir-143 in cervical specimens ranging from histologically normal through to invasive cervical cancer. PLoS One. 2011, 6 (12): e28423-10.1371/journal.pone.0028423.PubMedCentralCrossRefPubMed

26.

Qiang R, Wang F, Shi LY, Liu M, Chen S, Wan HY, Li YX, Li X, Gao SY, Sun BC, Tang H: Plexin-B1 is a target of miR-214 in cervical cancer and promotes the growth and invasion of HeLa cells. Int J Biochem Cell Biol. 2011, 43 (4): 632-641. 10.1016/j.biocel.2011.01.002.CrossRefPubMed

27.

Yang JH, Li JH, Shao P, Zhou H, Chen YQ, Qu LH: starBase: a database for exploring microRNA-mRNA interaction maps from Argonaute CLIP-Seq and Degradome-Seq data. Nucleic Acids Res. 2011, 39: D202-D209. 10.1093/nar/gkq1056.PubMedCentralCrossRefPubMed

28.

Dennis GJ, Sherman BT, Hosack DA, Yang J, Gao W, Lane HC, Lempicki RA: DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biol. 2003, 4 (5): 3-10.1186/gb-2003-4-5-p3.CrossRef

29.

Kahlert S, Nuedling S, van Eickels M, Vetter H, Meyer R, Grohe C: Estrogen receptor alpha rapidly activates the IGF-1 receptor pathway. J Biol Chem. 2000, 275 (24): 18447-18453. 10.1074/jbc.M910345199.CrossRefPubMed

30.

Lengyel F, Vertes Z, Kovacs KA, Kornyei JL, Sumegi B, Vertes M: Effect of estrogen and inhibition of phosphatidylinositol-3 kinase on Akt and FOXO1 in rat uterus. Steroids. 2007, 72 (5): 422-428. 10.1016/j.steroids.2007.03.001.CrossRefPubMed

31.

Brake T, Lambert PF: Estrogen contributes to the onset, persistence, and malignant progression of cervical cancer in a human papillomavirus-transgenic mouse model. Proc Natl Acad Sci U S A. 2005, 102 (7): 2490-2495. 10.1073/pnas.0409883102.PubMedCentralCrossRefPubMed

32.

Josson S, Anderson CS, Sung SY, Johnstone PA, Kubo H, Hsieh CL, Arnold R, Gururajan M, Yates C, Chung LW: Inhibition of ADAM9 expression induces epithelial phenotypic alterations and sensitizes human prostate cancer cells to radiation and chemotherapy. Prostate. 2011, 71 (3): 232-240. 10.1002/pros.21237.PubMedCentralCrossRefPubMed

33.

Zubel A, Flechtenmacher C, Edler L, Alonso A: Expression of ADAM9 in CIN3 lesions and squamous cell carcinomas of the cervix. Gynecol Oncol. 2009, 114 (2): 332-336. 10.1016/j.ygyno.2009.05.005.CrossRefPubMed

34.

Abdel-Fatah TM, Perry C, Dickinson P, Ball G, Moseley P, Madhusudan S, Ellis IO, Chan SY: Bcl2 is an independent prognostic marker of triple negative breast cancer (TNBC) and predicts response to anthracycline combination (ATC) chemotherapy (CT) in adjuvant and neoadjuvant settings. Ann Oncol. 2013, 24 (11): 2801-2807. 10.1093/annonc/mdt277.CrossRefPubMed

35.

Hagemann T, Bozanovic T, Hooper S, Ljubic A, Slettenaar VI, Wilson JL, Singh N, Gayther SA, Shepherd JH, Van Trappen PO: Molecular profiling of cervical cancer progression. Br J Cancer. 2007, 96 (2): 321-328. 10.1038/sj.bjc.6603543.PubMedCentralCrossRefPubMed

36.

Yoon SM, Shin KH, Kim JY, Seo SS, Park SY, Moon SH, Cho KH: Use of serum squamous cell carcinoma antigen for follow-up monitoring of cervical cancer patients who were treated by concurrent chemoradiotherapy. Radiat Oncol. 2010, 5: 78-10.1186/1748-717X-5-78.PubMedCentralCrossRefPubMed

37.

Bender DP, Sorosky JI, Buller RE, Sood AK: Serum CA 125 is an independent prognostic factor in cervical adenocarcinoma. Am J Obstet Gynecol. 2003, 189 (1): 113-117. 10.1067/mob.2003.443.CrossRefPubMed

38.

Bachtiary B, Selzer E, Knocke TH, Potter R, Obermair A: Serum VEGF levels in patients undergoing primary radiotherapy for cervical cancer: impact on progression-free survival. Cancer Lett. 2002, 179 (2): 197-203. 10.1016/S0304-3835(01)00872-2.CrossRefPubMed

39.

Jin XW, Lipold L, McKenzie M, Sikon A: Cervical cancer screening: what's new and what's coming?. Cleve Clin J Med. 2013, 80 (3): 153-160. 10.3949/ccjm.80a.12092.CrossRefPubMed

40.

Ng EK, Chong WW, Jin H, Lam EK, Shin VY, Yu J, Poon TC, Ng SS, Sung JJ: Differential expression of microRNAs in plasma of patients with colorectal cancer: a potential marker for colorectal cancer screening. Gut. 2009, 58 (10): 1375-1381. 10.1136/gut.2008.167817.CrossRefPubMed

41.

Qi P, Cheng SQ, Wang H, Li N, Chen YF, Gao CF: Serum microRNAs as biomarkers for hepatocellular carcinoma in Chinese patients with chronic hepatitis B virus infection. PLoS One. 2011, 6 (12): e28486-10.1371/journal.pone.0028486.PubMedCentralCrossRefPubMed

Title: Differentially expressed microRNAs in the serum of cervical squamous cell carcinoma patients before and after surgery
Authors: Wen-Tao Wang
Ya-Nan Zhao
Jin-Xing Yan
Mei-Ying Weng
Yan Wang
Yue-Qin Chen
Shun-Jia Hong
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: Journal of Hematology & Oncology / Issue 1/2014
Electronic ISSN: 1756-8722
DOI: https://doi.org/10.1186/1756-8722-7-6

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