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Virology Journal

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Open Access 01-12-2014 | Research

CHD1 and CHD2 are positive regulators of HIV-1 gene expression

Authors: Melissa J Rodgers, David J Banks, Kenneth A Bradley, John AT Young

Published in: Virology Journal | Issue 1/2014

Abstract

Background

Retroviruses encode a very limited number of proteins and therefore must exploit a wide variety of host proteins for completion of their lifecycle.

Methods

We performed an insertional mutagenesis screen to identify novel cellular regulators of retroviral replication.

Results

This approach identified the ATP-dependent chromatin remodeler, chromodomain helicase DNA-binding protein 2 (CHD2), as well as the highly related CHD1 protein, as positive regulators of both MLV and HIV-1 replication in rodent and human cells. RNAi knockdown of either CHD2 or the related CHD1 protein, in human cells resulted in a block to infection by HIV-1, specifically at the level of transcription.

Conclusions

These results demonstrate that CHD1 and CHD2 can act as positive regulators of HIV-1 gene expression.

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Title: CHD1 and CHD2 are positive regulators of HIV-1 gene expression
Authors: Melissa J Rodgers
David J Banks
Kenneth A Bradley
John AT Young
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2014
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/1743-422X-11-180

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CHD1 and CHD2 are positive regulators of HIV-1 gene expression

Abstract

Background

Methods

Results

Conclusions

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Abstract

Background

Methods

Results

Conclusions

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