Abstract
Silencing induced by long terminal repeat (LTR)–encoded cis-acting response element, termed SILENCE, is a forward genetic system that allows for conditional, epigenetic control of host-gene transcription. This new research tool is independent of gene mutation or disruption, does not require complementation, and conditional gene repression appears complete at the level of protein function. SILENCE functions in hypodiploid cells and is a platform technology with broad applications in gene discovery.
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Acknowledgements
This work was supported by US National Institutes of Health awards AI-057870 and AI-59095. D.J.B. was supported by National Institutes of Health Microbial Pathogenesis Training Grant 2-T32-AI-07323. Flow cytometry was performed in the UCLA Jonsson Comprehensive Cancer Center (JCCC) and Center for AIDS Research Flow Cytometry Core Facility, which is supported by National Institutes of Health awards CA-16042 and AI-28697, and by the JCCC, the UCLA AIDS Institute and the David Geffen School of Medicine at UCLA. We thank P.J. Bradley for helpful suggestions and insight.
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Supplementary Table 1
Oligonucleotide sequences.
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Banks, D., Bradley, K. SILENCE: a new forward genetic technology. Nat Methods 4, 51–53 (2007). https://doi.org/10.1038/nmeth991
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DOI: https://doi.org/10.1038/nmeth991
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