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Journal of Neuroinflammation
Published in: Journal of Neuroinflammation 1/2013

Open Access 01-12-2013 | Research

Exercise reduces activation of microglia isolated from hippocampus and brain of aged mice

Authors: Rachel A Kohman, Tushar K Bhattacharya, Elzbieta Wojcik, Justin S Rhodes

Published in: Journal of Neuroinflammation | Issue 1/2013

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Abstract

Background

Aging is associated with low-grade neuroinflammation that includes basal increases in proinflammatory cytokines and expression of inflammatory markers on microglia. Exercise can reduce neuroinflammation following infection in aged animals, but whether exercise modulates basal changes in microglia activation is unknown. Therefore, we evaluated changes in basal microglia activation in cells isolated from the hippocampus and remaining brain following running-wheel access.

Methods

Adult (4 months) and aged (22 months) male and female BALB/c mice were housed with or without running wheels for 10 weeks. Microglia were isolated from the hippocampus or remaining brain. Flow cytometry was used to determine microglia (CD11b+ and CD45low) that co-labeled with CD86, CD206, and MHC II.

Results

Aged mice showed a greater proportion of CD86 and MHC II positive microglia. In aged females, access to a running wheel decreased proportion of CD86+ and MHC II+ microglia in the hippocampus whereas aged males in the running group showed a decrease in the proportion of CD86+ microglia in the brain and an increase in the proportion of MHC II+ microglia in hippocampus and brain.

Conclusion

Overall, these data indicate that running-wheel access modulates microglia activation, but these effects vary by age, sex, and brain region.
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Metadata
Title
Exercise reduces activation of microglia isolated from hippocampus and brain of aged mice
Authors
Rachel A Kohman
Tushar K Bhattacharya
Elzbieta Wojcik
Justin S Rhodes
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Journal of Neuroinflammation / Issue 1/2013
Electronic ISSN: 1742-2094
DOI
https://doi.org/10.1186/1742-2094-10-114

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