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Translational Stroke Research
Published in: Translational Stroke Research 2/2017

01-04-2017 | Original Article

Early Erythrolysis in the Hematoma After Experimental Intracerebral Hemorrhage

Authors: Ge Dang, Yuefan Yang, Gang Wu, Ya Hua, Richard F. Keep, Guohua Xi

Published in: Translational Stroke Research | Issue 2/2017

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Abstract

Erythrolysis occurs in the clot after intracerebral hemorrhage (ICH), and the release of hemoglobin causes brain injury, but it is unclear when such lysis occurs. The present study examined early erythrolysis in rats. ICH rats had an intracaudate injection of 100 μl autologous blood, and sham rats had a needle insertion. All rats had T2 and T2* magnetic response imaging (MRI) scanning, and brains were used for histology and CD163 (a hemoglobin scavenger receptor) and DARPP-32 (a neuronal marker) immunohistochemistry. There was marked heterogeneity within the hematoma on T2* MRI, with a hyperintense or isointense core and a hypointense periphery. Hematoxylin and eosin staining in the same animals showed significant erythrolysis in the core with the formation of erythrocyte ghosts. The degree of erythrolysis correlated with the severity of perihematomal neuronal loss. Perihematomal CD163 was increased by day 1 after ICH and may be involved in clearing hemoglobin caused by early hemolysis. Furthermore, ICH resulted in more severe erythrolysis, neuronal loss, and perihematomal CD163 upregulation in spontaneously hypertensive rats compared to Wistar-Kyoto rats. In conclusion, T2*MRI-detectable early erythrolysis occurred in the clot after ICH and activated CD163. Hypertension is associated with enhanced erythrolysis in the hematoma.
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Metadata
Title
Early Erythrolysis in the Hematoma After Experimental Intracerebral Hemorrhage
Authors
Ge Dang
Yuefan Yang
Gang Wu
Ya Hua
Richard F. Keep
Guohua Xi
Publication date
01-04-2017
Publisher
Springer US
Published in
Translational Stroke Research / Issue 2/2017
Print ISSN: 1868-4483
Electronic ISSN: 1868-601X
DOI
https://doi.org/10.1007/s12975-016-0505-3

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