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Translational Stroke Research
Published in: Translational Stroke Research 3/2015

01-06-2015 | Original Article

Iron-Induced Necrotic Brain Cell Death in Rats with Different Aerobic Capacity

Authors: Mingzhe Zheng, Hanjian Du, Wei Ni, Lauren G. Koch, Steven L. Britton, Richard F. Keep, Guohua Xi, Ya Hua

Published in: Translational Stroke Research | Issue 3/2015

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Abstract

Brain iron overload has a key role in brain injury after intracerebral hemorrhage (ICH). Our recent study demonstrated that ICH-induced brain injury was greater in low capacity runner (LCR) than in high capacity runner (HCR) rats. The present study examines whether iron-induced brain injury differs between LCRs and HCRs. Adult male LCR and HCR rats had an intracaudate injection of iron or saline. Rats were euthanized at 2 and at 24 h after T2 magnetic resonance imaging, and the brains were used for immunostaining and Western blotting. LCRs had more hemispheric swelling, T2 lesion volumes, blood-brain barrier disruption, and neuronal death at 24 h after iron injection (p < 0.05). Many propidium iodide (PI)-positive cells, indicative of necrotic cell death, were observed in the ipsilateral basal ganglia of both HCRs and LCRs at 2 h after iron injection. PI fluorescence intensity was higher in LCRs than in HCRs. In addition, membrane attack complex (MAC) expression was increased at 2 h after iron injection and was higher in LCRs than in HCRs. The PI-positive cells co-localized with MAC-positive cells in the ipsilateral basal ganglia. Iron induces more severe necrotic brain cell death, brain swelling, and blood-brain barrier disruption in LCR rats, which may be related with complement activation and MAC formation.
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Metadata
Title
Iron-Induced Necrotic Brain Cell Death in Rats with Different Aerobic Capacity
Authors
Mingzhe Zheng
Hanjian Du
Wei Ni
Lauren G. Koch
Steven L. Britton
Richard F. Keep
Guohua Xi
Ya Hua
Publication date
01-06-2015
Publisher
Springer US
Published in
Translational Stroke Research / Issue 3/2015
Print ISSN: 1868-4483
Electronic ISSN: 1868-601X
DOI
https://doi.org/10.1007/s12975-015-0388-8

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