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Journal of Cardiovascular Translational Research

Published in:

01-10-2018 | Original Article

Transplantation of Cardiac Mesenchymal Stem Cell-Derived Exosomes for Angiogenesis

Authors: Chengwei Ju, Youngjun Li, Yan Shen, Yutao Liu, Jingwen Cai, Naifeng Liu, Gengshan Ma, Yaoliang Tang

Published in: Journal of Cardiovascular Translational Research | Issue 5/2018

Abstract

We demonstrated the effects of exosomes secreted by cardiac mesenchymal stem cells (C-MSC-Exo) in protecting acute ischemic myocardium from reperfusion injury. To investigate the effect of exosomes from C-MSC on angiogenesis, we injected C-MSC-Exo or PBS intramuscularly into ischemic hind limb. Blood perfusion of limb was evaluated by laser Doppler Imaging. We observed that ischemic limb treated with C-MSC-Exo exhibits improved blood perfusion compared to ischemic limb treated with PBS at 2 weeks and 1 month after induction of limb ischemia. To explore the potential mechanisms underlying C-MSC-Exo’s angiogenetic effect, we performed microRNA array analysis and identify mmu-miR-7116-5p as the most abundant enriched miRNA detected in C-MSC-Exo. Bioinformatics’ analysis shows that miR-7116-5p negatively regulates protein polyubiquitination. In conclusion, our study demonstrated that intramuscular delivery of C-MSC-Exo after limb ischemia improves blood perfusion, and we identified the most abundant miRNAs that are preferentially enriched in C-MSC-Exo.

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Title: Transplantation of Cardiac Mesenchymal Stem Cell-Derived Exosomes for Angiogenesis
Authors: Chengwei Ju
Youngjun Li
Yan Shen
Yutao Liu
Jingwen Cai
Naifeng Liu
Gengshan Ma
Yaoliang Tang
Publication date: 01-10-2018
Publisher: Springer US
Published in: Journal of Cardiovascular Translational Research / Issue 5/2018
Print ISSN: 1937-5387
Electronic ISSN: 1937-5395
DOI: https://doi.org/10.1007/s12265-018-9824-y

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