Abstract
Cardiac progenitor cells (CPC) are a unique pool of progenitor cells residing in the heart that play an important role in cardiac homeostasis and physiological cardiovascular cell turnover during acute myocardial infarction (MI). Transplanting CPC into the heart has shown promise in two recent clinical trials of cardiac repair (SCIPIO & CADUCEUS). CSCs were originally isolated directly from enzymatically digested hearts followed by cell sorting using stem cell markers. However, long exposure to enzymatic digestion can affect the integrity of stem cell markers on the cell surface and also compromise stem cell function. Here, we describe a two-step procedure in which a large number of intact cardiac progenitor cells can be purified from small amount of heart tissue.
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References
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Acknowledgement
This work was supported by the American Heart Association Beginning Grant-in-Aid 0765094Y (to Y.T.), NIH grant HL086555 (to Y.T.), and NIH grants HL076684 and HL62984 (to N.L.W.).
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© 2013 Springer Science+Business Media New York
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Chen, L., Pan, Y., Zhang, L., Wang, Y., Weintraub, N., Tang, Y. (2013). Two-Step Protocol for Isolation and Culture of Cardiospheres. In: Kao, R. (eds) Cellular Cardiomyoplasty. Methods in Molecular Biology, vol 1036. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-511-8_6
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DOI: https://doi.org/10.1007/978-1-62703-511-8_6
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-510-1
Online ISBN: 978-1-62703-511-8
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