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Annals of Nuclear Medicine
Published in: Annals of Nuclear Medicine 9/2020

01-09-2020 | Computed Tomography | Original Article

Visualization of translocator protein (18 kDa) (TSPO) in the retina of diabetic retinopathy rats using fluorine-18-DPA-714

Authors: Yujing Zhou, Yinghui Ou, Zizhao Ju, Xiaoqing Zhang, Lingling Zheng, Jun Li, Yu Sun, Xingdang Liu

Published in: Annals of Nuclear Medicine | Issue 9/2020

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Abstract

Objectives

To investigate the feasibility of a noninvasive method for imaging translocator protein (18 kDa) (TSPO) in the retina of diabetic retinopathy (DR) rats using fluorine-18-DPA-714 ([18F]-DPA-714) micro-positron emission tomography (PET)/X-ray computed tomography (CT).

Methods

Sprague–Dawley (SD) rats were intraperitoneally injected with streptozocin (STZ) (65 mg kg−1, ip) to induce diabetes mellitus (DM). The TSPO in both eyes was detected by PET/CT using [18F]-DPA-714 12 weeks after the establishment of the DM model. The mean standardized uptake value (SUVmean) was analyzed. Western blot and quantitative real-time polymerase chain reaction (PCR) were performed to detect the TSPO protein and mRNA levels in the retina.

Results

PET/CT results showed that the SUV of [18F]-DPA-714 was markedly reduced in the retina of DR rats compared with that of normal controls 12 weeks after diabetes induction. The SUVmean of regions of interest (ROIs) in the retinas of DR and normal control rats was 0.883 ± 0.078 and 2.525 ± 0.213 (P < 0.001), respectively. The results of PET/CT were in line with the Western blots and quantitative real-time PCR.

Conclusions

The PET results demonstrated that TSPO was decreased in the early stage of DR. [18F]-DPA-714 PET/CT appears to be a useful noninvasive imaging method for detecting TSPO in the retina. A decrease in the TSPO level in the retina may play an important role in the development of DR.
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Metadata
Title
Visualization of translocator protein (18 kDa) (TSPO) in the retina of diabetic retinopathy rats using fluorine-18-DPA-714
Authors
Yujing Zhou
Yinghui Ou
Zizhao Ju
Xiaoqing Zhang
Lingling Zheng
Jun Li
Yu Sun
Xingdang Liu
Publication date
01-09-2020
Publisher
Springer Singapore
Published in
Annals of Nuclear Medicine / Issue 9/2020
Print ISSN: 0914-7187
Electronic ISSN: 1864-6433
DOI
https://doi.org/10.1007/s12149-020-01495-2

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