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Journal of Assisted Reproduction and Genetics

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Open Access 01-02-2019 | Embryo Biology

Limited relationships between reactive oxygen species levels in culture media and zygote and embryo development

Authors: Kuo-Chung Lan, Yi-Chi Lin, Yung-Chiao Chang, Hsin-Jung Lin, Yi-Ru Tsai, Hong-Yo Kang

Published in: Journal of Assisted Reproduction and Genetics | Issue 2/2019

Abstract

Purpose

Reactive oxygen species (ROS) are thought to play a critical role in the success of IVF. The relationships between oxidative stress parameters in culture media and IVF outcomes have not been extensively investigated. The objective of this study is to examine the relationships between early human embryonic parameters and levels of ROS in culture media.

Methods

This prospective study was conducted with 2633 spent culture media collected from patients undergoing conventional IVF (n = 101) and intracytoplasmic sperm injection (ICSI) (n = 60). Both fertilization and early culture were performed in universal IVF medium and G series medium. ROS levels were measured by chemiluminescence assays using luminol as the probe on days 1, 3, and 5 and determined the relationships of ROS levels with zygote condition, embryo quality, and clinical pregnancy rate.

Results

ROS levels per embryo in culture media on the corresponding days 1, 3, and 5 showed significant correlations between each pair in the total cohort. Similar results were observed in the IVF and ICSI groups, but day 1 and day 3 ROS levels were significantly higher in culture media of IVF than of ICSI embryos. ROS levels in culture medium were not significantly associated with embryo quality, blastocyst formation, or arrest. ROS levels on day 1 were similar in media of normally fertilized zygotes, unfertilized oocytes, and polyspermic zygotes and were not associated with delayed embryonic development, high fragmentation, blastocyst formation, or arrest after prolonged culture. ROS levels in media were not associated with the likelihood of conception.

Conclusions

ROS levels in culture media may not be an effective indicator of embryo selection for IVF.

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Title: Limited relationships between reactive oxygen species levels in culture media and zygote and embryo development
Authors: Kuo-Chung Lan
Yi-Chi Lin
Yung-Chiao Chang
Hsin-Jung Lin
Yi-Ru Tsai
Hong-Yo Kang
Publication date: 01-02-2019
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 2/2019
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-018-1363-6

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Limited relationships between reactive oxygen species levels in culture media and zygote and embryo development

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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