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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 2/2019

01-02-2019 | Embryo Biology

Time-lapse imaging reveals delayed development of embryos carrying unbalanced chromosomal translocations

Authors: Hadar Amir, Shiri Barbash-Hazan, Yael Kalma, Tsvia Frumkin, Mira Malcov, Nivin Samara, Joseph Hasson, Adi Reches, Foad Azem, Dalit Ben-Yosef

Published in: Journal of Assisted Reproduction and Genetics | Issue 2/2019

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Abstract

Purpose

The purpose of the study was to compare the morphokinetic parameters of embryos carrying balanced chromosomal translocations with those carrying unbalanced chromosomal translocations using time-lapse microscopy.

Methods

The study group included 270 embryos that underwent biopsies on day 3 for preimplantation genetic diagnosis (PGD) for chromosomal translocations in our unit between 2013 and 2015. All embryos were incubated under time-lapse microscopy and evaluated for timing of developmental events up to day 5. The timing of these events was compared between balanced and unbalanced embryos, potentially viable and nonviable variants, and maternal versus paternal inheritance of the translocation.

Results

The PGD analysis found that 209 (77%) of the 270 biopsied embryos carried an unbalanced translocation. Embryos carrying unbalanced translocations, which are expected to lead to implantation failure or miscarriage, cleaved less synchronously and were delayed in time of cleavage to the 4-cell stage (t4) and in time of start of blastulation (tSB) compared with balanced embryos (P < 0.05). Furthermore, embryos carrying nonviable translocations demonstrated a significant delay at the time of pronuclei fading (tPNf) compared with those carrying potentially viable translocations (P < 0.05). Embryos whose unbalanced translocations were of maternal origin were significantly delayed in most of the morphokinetic parameters (including tPNf, t2, t3, t4, t6, t7, t8, cc2, s2, and tSB) compared with embryos carrying balanced translocations (P < 0.05).

Conclusions

Embryos carrying unbalanced chromosomal translocations mainly of maternal origin undergo delayed development and asynchronous cleavage that may lead to implantation failure or miscarriage.
Appendix
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Metadata
Title
Time-lapse imaging reveals delayed development of embryos carrying unbalanced chromosomal translocations
Authors
Hadar Amir
Shiri Barbash-Hazan
Yael Kalma
Tsvia Frumkin
Mira Malcov
Nivin Samara
Joseph Hasson
Adi Reches
Foad Azem
Dalit Ben-Yosef
Publication date
01-02-2019
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 2/2019
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-018-1361-8

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