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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 2/2019

01-02-2019 | Gamete Biology

Supplementation of biotin to sperm preparation medium enhances fertilizing ability of spermatozoa and improves preimplantation embryo development

Authors: Sujith Raj Salian, Guruprasad Nayak, Sandhya Kumari, Sandesh Patel, Shruthi Gowda, Yashaswini Shenoy, Sinoy Sugunan, Rajanikant G.K, Renuka Suresh Managuli, Srinivas Mutalik, Vandana Dahiya, Samanwita Pal, Satish Kumar Adiga, Guruprasad Kalthur

Published in: Journal of Assisted Reproduction and Genetics | Issue 2/2019

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Abstract

Purpose

Motility of spermatozoa helps not only in planning the type of infertility treatment but also directly reflects the success rate in assisted reproductive technology (ART). Previously, biotin, a water-soluble vitamin, has been shown to increase the motility and longevity of cryopreserved human spermatozoa. The present study was designed to understand the molecular basis of the beneficial effects of presence of biotin in sperm wash medium on early embryo development.

Methods

The effect biotin supplementation to sperm wash medium on the sperm parameters were assessed in swim-up fraction of normozoospermic and asthenozoospermic ejaculates collected from infertile men. Fertilization and early embryo development was studied using Swiss albino mice.

Results

Even though both biotin and pentoxifylline (PTX) enhanced the motility of spermatozoa from normozoospermic and asthenozoospermic samples, biotin group exhibited higher in vitro survival. Using mouse model, we observed that presence of biotin or PTX in sperm wash medium improved the fertilization rate and blastocyst rate compared to control. Blastocysts from these groups had significantly higher total cell number (P < 0.01) and lower apoptotic index. In silico target prediction revealed that GTPase HRas (HRas), tyrosine-protein phosphatase nonreceptor type 1 (PTP1B), and glucokinase are the probable targets for biotin. Solution-state Nuclear Magnetic Resonance (NMR) studies confirmed that biotin interacts both with human HRas and PTP1B.

Conclusion

Our results indicate that presence of biotin in sperm wash medium can improve the fertilization potential and preimplantation embryo development and can be considered as a safe alternate to PTX.
Appendix
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Metadata
Title
Supplementation of biotin to sperm preparation medium enhances fertilizing ability of spermatozoa and improves preimplantation embryo development
Authors
Sujith Raj Salian
Guruprasad Nayak
Sandhya Kumari
Sandesh Patel
Shruthi Gowda
Yashaswini Shenoy
Sinoy Sugunan
Rajanikant G.K
Renuka Suresh Managuli
Srinivas Mutalik
Vandana Dahiya
Samanwita Pal
Satish Kumar Adiga
Guruprasad Kalthur
Publication date
01-02-2019
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 2/2019
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-018-1323-1

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