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International Ophthalmology

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Open Access 01-02-2020 | Strabismus | Original Paper

Intermediate filaments in the medial rectus muscles in patients with concomitant exotropia

Authors: Tao Shen, Jing Lin, Xiuling Li, Daming Deng

Published in: International Ophthalmology | Issue 2/2020

Abstract

Purpose

Distribution of intermediate filament (IF) proteins in normal extraocular muscles (EOMs) showed that the EOMs differ significantly from the other muscles in the body with respect to their IFs composition, including desmin and nestin. The aim of the present study was to investigate the pathological changes in the medial rectus (MR) in patients with concomitant exotropia (XT).

Methods

Forty-six MR muscle samples from 46 patients with XT were analyzed pathologically and processed for immunohistochemistry with specific antibodies against desmin and nestin.

Results

Although most of MR muscles remained normal structures relatively, they presented high expression of desmin, and in contrast, nestin was absent in a large proportion of the MR muscles.

Conclusion

Desmin, which is downregulated in normal EOMs, had high expression in MR muscles of patients with XT. Nestin, which is present in a high proportion of normal EOMs, was downregulated in MR muscles of patients with XT.

Arora A, Williams B, Arora AK, McNamara R, Yates J, Fielder A (2005) Decreasing strabismus surgery. Br J Ophthalmol 89(4):409–412. https://doi.org/10.1136/bjo.2004.053678 CrossRefPubMedPubMedCentral

Rabinowitz R, Demer JL (2014) Muscle path length in horizontal strabismus. J AAPOS 18(1):4–9. https://doi.org/10.1016/j.jaapos.2013.10.019 CrossRefPubMedPubMedCentral

Kjellgren D, Thornell LE, Andersen J, Pedrosa-Domellof F (2003) Myosin heavy chain isoforms in human extraocular muscles. Invest Ophthalmol Vis Sci 44(4):1419–1425CrossRef

Pedrosa-Domellof F, Holmgren Y, Lucas CA, Hoh JF, Thornell LE (2000) Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation. Invest Ophthalmol Vis Sci 41(7):1608–1616PubMed

Kjellgren D, Thornell LE, Virtanen I, Pedrosa-Domellof F (2004) Laminin isoforms in human extraocular muscles. Invest Ophthalmol Vis Sci 45(12):4233–4239. https://doi.org/10.1167/iovs.04-0456 CrossRefPubMed

Liu JX, Willison HJ, Pedrosa-Domellof F (2009) Immunolocalization of GQ1b and related gangliosides in human extraocular neuromuscular junctions and muscle spindles. Invest Ophthalmol Vis Sci 50(7):3226–3232. https://doi.org/10.1167/iovs.08-3333 CrossRefPubMed

Zhu Y, Deng D, Long C, Jin G, Zhang Q, Shen H (2013) Abnormal expression of seven myogenesis-related genes in extraocular muscles of patients with concomitant strabismus. Mol Med Rep 7(1):217–222. https://doi.org/10.3892/mmr.2012.1149 CrossRefPubMed

Janbaz AH, Lindstrom M, Liu JX, Pedrosa Domellof F (2014) Intermediate filaments in the human extraocular muscles. Invest Ophthalmol Vis Sci 55(8):5151–5159. https://doi.org/10.1167/iovs.14-14316 CrossRefPubMed

Demer JL, Oh SY, Clark RA, Poukens V (2003) Evidence for a pulley of the inferior oblique muscle. Invest Ophthalmol Vis Sci 44(9):3856–3865CrossRef

10.

Demer JL, Miller JM, Poukens V, Vinters HV, Glasgow BJ (1995) Evidence for fibromuscular pulleys of the recti extraocular muscles. Invest Ophthalmol Vis Sci 36(6):1125–1136PubMed

11.

Gralek M, Krawczyk T (1998) Pathomorphological evaluation of the extraocular muscles during strabismus. Klin Oczna 100(6):373–375PubMed

12.

Kim SH, Cho YA, Park CH, Uhm CS (2008) The ultrastructural changes of tendon axonal profiles of medial rectus muscles according to duration in patients with intermittent exotropia. Eye 22(8):1076–1081. https://doi.org/10.1038/eye.2008.133 CrossRefPubMed

13.

Li H, Choudhary SK, Milner DJ, Munir MI, Kuisk IR, Capetanaki Y (1994) Inhibition of desmin expression blocks myoblast fusion and interferes with the myogenic regulators MyoD and myogenin. J Cell Biol 124(5):827–841CrossRef

14.

Weitzer G, Milner DJ, Kim JU, Bradley A, Capetanaki Y (1995) Cytoskeletal control of myogenesis: a desmin null mutation blocks the myogenic pathway during embryonic stem cell differentiation. Dev Biol 172(2):422–439. https://doi.org/10.1006/dbio.1995.8070 CrossRefPubMed

15.

Milner DJ, Mavroidis M, Weisleder N, Capetanaki Y (2000) Desmin cytoskeleton linked to muscle mitochondrial distribution and respiratory function. J Cell Biol 150(6):1283–1298CrossRef

16.

Kachinsky AM, Dominov JA, Miller JB (1994) Myogenesis and the intermediate filament protein, nestin. Dev Biol 165(1):216–228. https://doi.org/10.1006/dbio.1994.1248 CrossRefPubMed

17.

Albers K, Fuchs E (1992) The molecular biology of intermediate filament proteins. Int Rev Cytol 134:243–279CrossRef

18.

Sejersen T, Lendahl U (1993) Transient expression of the intermediate filament nestin during skeletal muscle development. J Cell Sci 106(Pt 4):1291–1300PubMed

19.

Singh SK, Clarke ID, Hide T, Dirks PB (2004) Cancer stem cells in nervous system tumors. Oncogene 23(43):7267–7273. https://doi.org/10.1038/sj.onc.1207946 CrossRefPubMed

20.

Ryuge S, Sato Y, Wang GQ, Matsumoto T, Jiang SX, Katono K, Inoue H, Satoh Y, Masuda N (2011) Prognostic significance of nestin expression in resected non-small cell lung cancer. Chest 139(4):862–869. https://doi.org/10.1378/chest.10-1121 CrossRefPubMed

21.

Awadein A, Eltanamly RM, Elshazly M (2014) Intermittent exotropia: relation between age and surgical outcome: a change-point analysis. Eye 28(5):587–593. https://doi.org/10.1038/eye.2014.29 CrossRefPubMedPubMedCentral

Title: Intermediate filaments in the medial rectus muscles in patients with concomitant exotropia
Authors: Tao Shen
Jing Lin
Xiuling Li
Daming Deng
Publication date: 01-02-2020
Publisher: Springer Netherlands
Keywords: Strabismus
Strabismus
Published in: International Ophthalmology / Issue 2/2020
Print ISSN: 0165-5701
Electronic ISSN: 1573-2630
DOI: https://doi.org/10.1007/s10792-019-01197-3

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Intermediate filaments in the medial rectus muscles in patients with concomitant exotropia

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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