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Inflammation
Published in: Inflammation 3/2018

01-06-2018 | Original Article

Protective Effect of Quercetin in LPS-Induced Murine Acute Lung Injury Mediated by cAMP-Epac Pathway

Authors: Xue-feng Wang, Shun-de Song, Ya-jun Li, Zheng qiang Hu, Zhe-wen Zhang, Chun-guang Yan, Zi-gang Li, Hui-fang Tang

Published in: Inflammation | Issue 3/2018

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Abstract

Quercetin (Que) as an abundant flavonol element possesses potent antioxidative properties and has protective effect in lipopolysaccharide (LPS)-induced acute lung injury (ALI), but the specific mechanism is still unclear, so we investigated the effect of Que from in vivo and in vitro studies and the related mechanism of cAMP-PKA/Epac pathway. The results in mice suggested that Que can inhibit the release of inflammatory cytokine, block neutrophil recruitment, and decrease the albumin leakage in dose-dependent manners. At the same time, Que can increase the cAMP content of lung tissue, and Epac content, except PKA. The results in epithelial cell (MLE-12) suggested that Que also can inhibit the inflammatory mediators keratinocyte-derived chemokines release after LPS stimulation; Epac inhibitor ESI-09 functionally antagonizes the inhibitory effect of Que; meanwhile, PKA inhibitor H89 functionally enhances the inhibitory effect of Que. Overexpression of Epac1 in MLE-12 suggested that Epac1 enhance the effect of Que. All those results suggested that the protective effect of quercetin in ALI is involved in cAMP-Epac pathway.
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Metadata
Title
Protective Effect of Quercetin in LPS-Induced Murine Acute Lung Injury Mediated by cAMP-Epac Pathway
Authors
Xue-feng Wang
Shun-de Song
Ya-jun Li
Zheng qiang Hu
Zhe-wen Zhang
Chun-guang Yan
Zi-gang Li
Hui-fang Tang
Publication date
01-06-2018
Publisher
Springer US
Published in
Inflammation / Issue 3/2018
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI
https://doi.org/10.1007/s10753-018-0761-3

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