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The therapeutic effects of anti-oxidant and anti-inflammatory drug quercetin on aspiration-induced lung injury in rats

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Abstract

Aspiration pneumonitis refers to acute chemical lung injury caused by aspiration of sterile gastric contents. The aim of this study was to evaluate the role of quercetin (QC) in acid aspiration-induced lung injury in rats. Twenty-eight female Sprague–Dawley rats were used and divided into the following groups (n = 7): sham (aspirated normal saline, S), hydrochloric acid (aspirated HCl), S plus treatment with QC (S + QC), and HCl plus treatment with QC (HCl + QC). After aspiration, the treatment groups received QC 60 mg/kg/day intraperitoneally once a day for 7 days. As a result of acid aspiration, an increase was observed in the levels of serum clara cell protein-16 (CC-16) and advanced oxidation protein products, whereas there was a decrease in serum thiobarbituric acid-reactive substances, superoxide dismutase (SOD), and catalase levels. There was a significant decrease in peribronchial inflammatory cell infiltration, alveolar septal infiltration, alveolar edema, and alveolar exudate scores, except in the alveolar histiocytes in the HCl + QC group. The expression of nitric oxide synthase, which increased after aspiration in the HCl group, showed a statistically significant decrease after the QC treatment. After the treatment with QC, an increase in the serum SOD level was observed, whereas a significant decrease was determined in the serum CC-16 level relative to that of the aspiration group (HCl). The antioxidant QC is effective in the treatment of lung injury following acid aspiration and can be used as a serum CC-16 biomarker in predicting the severity of oxidative lung injury.

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Authors and Affiliations

  1. Department of Pedodontia, Faculty of Dentistry, Ondokuz Mayis University, Samsun, Turkey

    Mehmet Ziya Yilmaz & Aysun Caglar Torun

  2. Department of Chest Disease, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey

    Aygul Guzel

  3. Department of Medical Biochemistry, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey

    Ali Okuyucu & Hasan Alacam

  4. Samsun Highschool of Health, Ondokuz Mayis University, Samsun, Turkey

    Osman Salis

  5. Department of Otorhinolaryngology, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey

    Rifat Karli

  6. Department of Pathology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey

    Ayhan Gacar & Tolga Guvenc

  7. Department of Pediatrics, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey

    Sule Paksu

  8. Cizre State Hospital, Şırnak, Turkey

    Volkan Urey

  9. Industrial Engineering, Faculty of Engineering, Ondokuz Mayis University, Samsun, Turkey

    Naci Murat

Authors
  1. Mehmet Ziya Yilmaz
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  2. Aygul Guzel
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  3. Aysun Caglar Torun
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  4. Ali Okuyucu
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  7. Ayhan Gacar
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  8. Tolga Guvenc
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  9. Sule Paksu
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  10. Volkan Urey
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  11. Naci Murat
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  12. Hasan Alacam
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Correspondence to Mehmet Ziya Yilmaz.

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Yilmaz, M.Z., Guzel, A., Torun, A.C. et al. The therapeutic effects of anti-oxidant and anti-inflammatory drug quercetin on aspiration-induced lung injury in rats. J Mol Hist 45, 195–203 (2014). https://doi.org/10.1007/s10735-013-9542-3

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  • DOI: https://doi.org/10.1007/s10735-013-9542-3

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