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Magnetic Resonance Materials in Physics, Biology and Medicine

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01-06-2019 | Magnetic Resonance Imaging | Research Article

Challenges for labeling and longitudinal tracking of adoptively transferred autoreactive T lymphocytes in an experimental type-1 diabetes model

Authors: Shweta Saini, Hannelie Korf, Sayuan Liang, Rein Verbeke, Bella Manshian, Koen Raemdonck, Ine Lentacker, Conny Gysemans, Stefaan C. De Smedt, Uwe Himmelreich

Published in: Magnetic Resonance Materials in Physics, Biology and Medicine | Issue 3/2019

Abstract

Objective

Tracking the autoreactive T-cell migration in the pancreatic region after labeling with fluorinated nanoparticles (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[3-(2-pyridyldithio)propionate]-perfluoro-15-crown-5-ether nanoparticles, PDP-PFCE NPs) in a diabetic murine model using ¹⁹F MRI.

Materials and methods

Synthesis of novel PDP-PFCE fluorine tracer was performed for in vitro labeling of T cells. Labeling conditions were optimized using different PDP-PFCE NPs concentrations. For in vivo ¹⁹F MRI, mice were longitudinally followed after adoptive transfer of activated, autoreactive, labeled T cells in NOD.SCID mice.

Results

Established MR protocols were used for challenging T cell labeling to track inflammation in a model of diabetes after successful labeling of CD4+ and CD8+ T cells with PDP-PFCE NPs. However, T cells were difficult to be detected in vivo after their engraftment in animals.

Discussion

We showed successful in vitro labeling of T cells using novel fluorinated liposomal nanoparticles. However, insufficient and slow accumulation of labeled T cells and subsequent T cell proliferation in the pancreatic region remains as limitations of in vivo cell imaging by ¹⁹F MRI.

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Title: Challenges for labeling and longitudinal tracking of adoptively transferred autoreactive T lymphocytes in an experimental type-1 diabetes model
Authors: Shweta Saini
Hannelie Korf
Sayuan Liang
Rein Verbeke
Bella Manshian
Koen Raemdonck
Ine Lentacker
Conny Gysemans
Stefaan C. De Smedt
Uwe Himmelreich
Publication date: 01-06-2019
Publisher: Springer International Publishing
Keywords: Magnetic Resonance Imaging
Magnetic Resonance Imaging
Type 1 Diabetes
Published in: Magnetic Resonance Materials in Physics, Biology and Medicine / Issue 3/2019
Print ISSN: 0968-5243
Electronic ISSN: 1352-8661
DOI: https://doi.org/10.1007/s10334-018-0720-x

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Challenges for labeling and longitudinal tracking of adoptively transferred autoreactive T lymphocytes in an experimental type-1 diabetes model

Abstract

Objective

Materials and methods

Results

Discussion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Objective

Materials and methods

Results

Discussion

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